穿心莲中药血清联合抗菌药对含fosA3大肠杆菌的作用研究  

Study on the Effect of the Serum of Andrographis Herba Combined with Antibacterial Agents on the Fosfomycin Resistance Gene fosA3 in Escherichia coli

作　　者：吴永继 王鹏霞[1] 宋剑武[1] 刘增援 孙燕杰[1] 周大同[1] 司红彬[1] 

机构地区：[1]广西大学动物科学技术学院,广西南宁530005

出　　处：《中国兽医杂志》2015年第9期64-67,共4页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金(31460675);广西自然科学基金项目(2012GXNSFBA053052;2013GXNSFAA019070);广西大学动物科学技术学院科研发展基金项目(DK201105);广西大学兽医一级学科博士启动基金项目(2012GXNSFBA053052)

摘　　要：为了探讨穿心莲中药血清与抗菌药联合体外抗含耐磷霉素基因fos A3(Fosfomycin Resistance Gene)大肠杆菌的效果,对临床分离的细菌进行鉴定,确定细菌所含耐药基因种类,采用二倍微量稀释法分别体外检测穿心莲水提取物,中药血清与抗菌药物的最小抑菌浓度(MIC),并以1/2 MIC的穿心莲中药血清与抗菌药联合诱导含fos A3大肠杆菌的传代试验。结果显示,分离的菌株为含fos A3大肠杆菌;穿心莲水提物的MIC为1.0 g/m L,中药血清的MIC为1.0 m L/m L,以0.5 m L/m L的穿心莲中药血清与头孢曲松钠、阿莫西林、诺氟沙星、粘杆菌素、痢菌净、磺胺间甲氧嘧啶、阿米卡星、头孢他啶、头孢噻呋钠、氟苯尼考和磷霉素等11种抗菌药联合诱导传代后MIC显著降低。表明穿心莲中药血清联合抗菌药体外诱导含fos A3大肠杆菌,抗菌活性明显增强,并对含fos A3型大肠杆菌具有一定的耐药逆转作用。This study was aimed to investigate the effect of the serum of Andrographis Herba combined with antibiotics againstbacterial carrying fosfomycin resistance gene fos A3 in vitro.Resistance gene types were determined by identification of bacteria iso-lated in clinics. The minimum inhibitory concentration（MIC）of water extracts,the serum of Andrographis Herba and antibacterialagents were tested through twice micro-dilution method. fos A3 in Escherichia coli. was induced and subcultured by the 1/2 MIC ofthe serum of Andrographis Herba combined with antibacterial agents. The results showed that Escheriohia coli. carrying multidrugresistance gene fos A3 was isolated.The MIC of water extracts was 1.0 g/m L,and the serum of Andrographis Herba was 1.0 m L/m L.The MIC of fos A3 in Escherichia coli.was significantly decreased after inducing by 0.5 m L/m L of the serum of Andrographis Herbacombined with 11 antibacterial agents,such as Ceftriaxone Sodium,Amoxicillin,Norfloxacin,Coly-mycin,Mequindox,Sulfamono-methoxine,Amikacin,Ceftazidime,Ceftiofur sodium,and Florfenicol,Fosfomycin. It was concluded that antibacterial activities ofantibacterial agents against fos A3 in Escherichia coli. were significantly enhanced by the serum of Andrographis Herba,showingmultidrug resistance reversal effect.

关 键 词：穿心莲 fosA3大肠杆菌 联合诱导 最小抑菌浓度(MIC) 中药血清 

分 类 号：R282[医药卫生—中药学]