Effect of electronic stimulation at Neiguan(PC 6) acupoint on gene expression of adenosine triphosphate-sensitive potassium channel and protein kinases in rats with myocardial ischemia  

作　　者：Wang Wei Li Jiquan Meng Xiangyu Chen Yiguo 

机构地区：[1]College of Acupuncture and Massage,Liaoning University of Traditional Chinese Medicine

出　　处：《Journal of Traditional Chinese Medicine》2015年第5期577-582,共6页中医杂志（英文版）

基　　金：Supported by National Basic Research Program of China(973 Program);Study on Biological Foundation of Response in Target Organ to Meridian Specificity of Acupoint Effect(No.2012CB518503)

摘　　要：OBJECTIVE:To investigate the effects of electronic stimulation at acupoints Neiguan(PC 6) and Lieque(LU 7) on the gene expression of the adenosine triphosphate(ATP)-Sensitive potassium channel(KATP:Kir6.1,Kir6.2,SUR2 A,and SUR2B) and protein kinases(PKA,PKG,and PKCβ2) in myocardial cells of rats with myocardial ischemia(Ml) induced by isoproterenol(ISO).METHODS:Rats were randomly divided into a control,model,Neiguan(PC 6),Lieque(LU 7),and non-acupoint groups.The Ml model was established by injecting rats with ISO.Electro-acupuncture treatment was given to the acupuncture groups,once a day for 7 days.Gene expression was analyzed with real-time PCR.RESULTS:The gene expression of KATP and protein kinases in the model group was higher than those in the control group(P < 0.05).After acupuncture treatment,the KATP and protein kinase expression levels were significantly lower in the Neiguan(PC 6)and Lieque(LU 7) groups compared with the model group[P < 0.05).The Neiguan(PC 6) group lowered these levels significantly more than that of the Lieque(LU 7) group(P < 0.05).No significant differences were observed between the model and non-acupoint groups(P > 0.05).CONCLUSION:Our findings suggest that electronic needling of Neiguan(PC 6) can both reduce the gene expression of KATP and protein kinases in rats with ISO-induced Ml.OBJECTIVE：To investigate the effects of electronic stimulation at acupoints Neiguan（PC 6） and Lieque（LU 7） on the gene expression of the adenosine triphosphate（ATP）-Sensitive potassium channel（KATP：Kir6.1,Kir6.2,SUR2 A,and SUR2B） and protein kinases（PKA,PKG,and PKCβ2） in myocardial cells of rats with myocardial ischemia（Ml） induced by isoproterenol（ISO）.METHODS：Rats were randomly divided into a control,model,Neiguan（PC 6）,Lieque（LU 7）,and non-acupoint groups.The Ml model was established by injecting rats with ISO.Electro-acupuncture treatment was given to the acupuncture groups,once a day for 7 days.Gene expression was analyzed with real-time PCR.RESULTS：The gene expression of KATP and protein kinases in the model group was higher than those in the control group（P 〈 0.05）.After acupuncture treatment,the KATP and protein kinase expression levels were significantly lower in the Neiguan（PC 6）and Lieque（LU 7） groups compared with the model group[P 〈 0.05）.The Neiguan（PC 6） group lowered these levels significantly more than that of the Lieque（LU 7） group（P 〈 0.05）.No significant differences were observed between the model and non-acupoint groups（P 〉 0.05）.CONCLUSION：Our findings suggest that electronic needling of Neiguan（PC 6） can both reduce the gene expression of KATP and protein kinases in rats with ISO-induced Ml.

关 键 词：Point PC6（Neiguan） Acupuncture Electric stimulation KATP Channels Protein Kinases Myocardial ischemia 

分 类 号：R245[医药卫生—针灸推拿学]