Effect of short-term acumagnetotherapy on diabetic kidney disease in patients with type Ⅱ diabetes and study on the molecular mechanism  

短期穴位磁疗干预2型糖尿病肾病作用及其分子机制探讨（英文）

作　　者：周子瑜[1] 任丽伟[1] 杨含艳 刘继章[2] 褚克丹[1] 翁建平[2] 于志文[1] 

机构地区：[1]福建中医药大学生物医药研发中心 [2]中山大学第三附属医院内分泌科

出　　处：《World Journal of Acupuncture-Moxibustion》2015年第3期1-10,共10页世界针灸杂志（英文版）

基　　金：Supported by the Natural Science Foundation of China:81270886

摘　　要：Objective To explore whether magnetotherapy is preventive in retarding diabetic kidney disease（DKD） progression and investigate underling molecular mechanisms related to its therapeutic efficacy. Methods Twenty-eight patients with type Ⅱ diabetes（T2D） were undergone pulsed electromagnetic fields（PEMF） stimulation at the acupoints of Píshū（脾俞 BL 20）, Zúsānl（足三里 ST 36）, Shènshū（肾俞 BL 23） and Yíshū（胰俞, EX-B3） for a period of 4 weeks. Urinary micro-albumin（U-m Alb） excretion, plasma methane dicarboxylic aldehyde（MDA and lipopolysaccharide（LPS） of the patients were used for evaluating therapeutic efficacies. Results After the acumagnetotherapy, U-m Alb excretion in the participated patients was markedly reduced（27.21±3.51 vs 8.51±0.95, P0.001） accompanied with decreased MDA（16.46±1.17 vs 12.40±1.86, P0.05） and LPS（37.41±3.84 vs 21.63±3.61, P0.05） levels in plasma while the metabolic control of these patients was not significantly altered. Acumagnetotherapy increased IκBα content（0.69±1.17 vs 1.30±0.29, P0.01）, an inhibitory protein of inflammatory response, and correspondingly reduced the protein levels of inflammatory activating proteins, NF-κB p65（0.98±0.42 vs 0.43±0.28, P0.05） and NF-κB p50（1.19±0.40 vs 0.76±0.30, P0.05）. The acumagnetotherapy also inhibited the oxidantproducing enzyme, Nox4 protein expression（1.32±0.40 vs 0.37±0.23, P0.05） in patient ＇s blood lymphocytes. Conclusion Short-term intervention of acumagnetotherapy in patients with T2 D mitigates DKD progress potentially by its anti-oxidative and anti-inflammatory effects.目的:探讨穴位磁疗法是否能够延缓糖尿病肾病(DKD)的发展,并研究这一治疗作用的分子机制。方法:对28例2型糖尿病(T2D)患者在脾俞(BL 20)、足三里(ST 36)、肾俞(BL 23)和胰俞等穴位进行为期4周的脉冲磁疗干预,以病人尿微量白蛋白排出量、血浆丙二醛含量和脂多糖水平作为评价疗效的指标。结果:磁疗干预后,病人尿微量白蛋白排出量较干预前明显下降(27.21±3.51 vs8.51±0.95,P<0.001),血浆丙二醛明显降低(MDA,16.46±1.17 vs 12.40±1.86,P<0.05),脂多糖水平明显下降(LPS,37.41±3.84 vs 21.63±3.61,P<0.05),而其他代谢相关指标没有明显变化;磁疗法干预能够升高IκBα——炎症信号抑制蛋白含量(0.69±1.17 vs 1.30±0.29,P<0.01),并相应地降低炎症激活蛋白NF-κB p65(0.98±0.42 vs 0.43±0.28,P<0.05)和NF-κB p50(1.19±0.40 vs 0.76±0.30,P<0.05)的含量,磁疗干预还能够抑制氧化酶Nox4(1.32±0.40 vs 0.37±0.23,P<0.05)的表达。结论:短期磁疗干预T2D患者通过其潜在的抗氧化和抗炎作用延缓DKD发展。

关 键 词：acumagnetotherapy diabetic nephropathy urinary microalbumin oxidative stress LYMPHOCYTE 

分 类 号：R246.1[医药卫生—针灸推拿学]