Effect of acupuncture combined with Chinese medicine on the expression of interstitial cells of Cajal, substance P and nerve nitric oxide synthase in diabetic mice with gastroparesis antrum  

针药结合对糖尿病胃轻瘫小鼠胃窦Cajal间质细胞及SP、nNOS表达的影响（英文）

作　　者：喻欢[1] 程军平[2] 张定棋 唐翠娟[1] 黄坤艳[1] 谭凌菁 杨松柏[1] 梅志刚[1,3] 

机构地区：[1]三峡大学医学院 [2]苏州市中医医院 [3]三峡大学中医临床医学院宜昌市中医医院

出　　处：《World Journal of Acupuncture-Moxibustion》2015年第3期35-42,共8页世界针灸杂志（英文版）

基　　金：Supported by Suzhou Science and Technology Bureau Project:SYS 201365

摘　　要：Objective To explore the effect of acupuncture combined with Chinese medicine on expression of interstitial cells of Cajal（ICC）, substance P（SP） and nerve nitric oxide synthase（nNOS） in diabetic gastroparesis mice. Methods Eighty Kunming male mice were randomly divided into normal group（group A, 15 mice）, diabetic gastroparesis model mice group（group B, 12 mice）, acupuncture combined with Chinese medicine group（group C, 12 mice）, Chinese medicine group（group D, 13 mice） and western medicine group（group E, 13 mice）. The mice were intraperitoneally injected with steptozotocin and fed with high-fat diet-induced irregular except the normal group mice, that made mouse model of diabetic gastroparesis. And then, the ACM group were treated by acupuncture at the acupoints of Housanli（后三里 ST 36） and Zhōngw n（中脘 CV 12） combined with Chinese medicine, the CM group were only treated by Chinese medicine; the WM group were treated by domperidone and vitamin B 6. Numbers and expression of ICC, SP and n NOS were detected by immunohistochemical methods. Results After treatment, compared with normal group, ICC and SP indexes of model group were significantly reduced. Compared with model group, all indexes in ACM and CM groups were improved significantly（P0.05, P0.01）, but only the positive area and average optical density value in c-Kit and SP in WM group increased obviously（P0.05）. Compared with ACM group, no significant difference in c-Kit and SP of CM group were observed（P0.05）, but expression of nNOS were increased markedly（P0.01）. Besides, c-Kit and SP expressions of WM group were significantly induced was increased remarkably（P0.01）. Conclusion Acupuncture combined with Chinese medicine have much better therapeutic effects for diabetic gastroparesis mice, which may be due to repairing ICC damage, upregulating the expression of SP and lowering the expression of nNOS.目的:研究针药结合对糖尿病胃轻瘫小鼠胃窦Cajal间质细胞(ICC)、P物质(SP)及神经性一氧化氮合酶(n NOS)表达的影响。方法:80只雄性昆明小鼠随机分为正常组(15只)、模型组(12只)、针药组(12只)、中药组(13只)和西药组(13只)。除正常组外,一次性腹腔注射链脲佐菌素并高糖高脂喂养,诱导糖尿病胃轻瘫模型。针药组采取"后三里""中脘"电针合并中药方糖瘫消灌胃(0.026 m L/g,1次/d);中药组仅予以糖瘫消灌胃治疗;西药组予以多潘立酮和维生素B 6混合液灌胃。采用免疫组化法检测并比较各组小鼠胃窦部ICC数量、SP和n NOS含量及其分布。结果:与正常组比较,模型组ICC、SP各指标均显著减少,n NOS指标均显著增加(P<0.01);治疗后,与模型组比较,针药组和中药组各指标均显著性改善(P<0.05或P<0.01),而西药组ICC、SP仅有阳性面积百分比、平均光密度值指标有显著性升高(P<0.05);与针药组比较,治疗后中药组ICC、SP等指标差异无统计学意义(P>0.05),n NOS仅单位面积阳性细胞数均显著降低,n NOS各指标显著升高(P<0.01)。结论:针药结合对糖尿病胃轻瘫模型小鼠有较好的治疗效果,其机制可能与恢复ICC的损伤、上调SP表达,下调n NOS表达有关。

关 键 词：acupuncture combined with Chinese medicine diabetic gastroparesis ICC SP nNOS 

分 类 号：R245[医药卫生—针灸推拿学]