实时荧光定量PCR技术检测肺炎衣原体的诊断价值  

Diagnosis of chlamydiales pneumoniae using real-time quantitative PCR

作　　者：罗焰芳[1] 赵林[1] 李友琼[1] 黄驾 谢超冰 

机构地区：[1]广西壮族自治区人民医院检验科,南宁530021 [2]百色市田东县人民医院检验科,广西531500 [3]贵港市平南县第二人民医院检验科,广西537300

出　　处：《中国临床新医学》2015年第10期962-964,共3页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

摘　　要：目的探讨实时荧光定量PCR技术检测肺炎衣原体的诊断价值。方法对呼吸道感染的186例患者的痰液分别采用实时荧光定量PCR技术和基因测序检测,以评价实时荧光定量PCR技术检测肺炎衣原体感染的准确性。结果实时荧光定量PCR检测肺炎衣原体的灵敏度为100.0%,特异度为93.1%,阳性预期值为80.8%,阴性预期值为100.0%,准确率为94.6%。结论实时荧光定量PCR技术检测肺炎衣原体具有较高的可靠性,适用于临床快速诊断肺炎衣原体感染的相关疾病。Objective To study the diagnostic value of real-time quantitative PCR detection in chlamydiales pneumoniae. Methods Sputum samples from 186 patients with respiratory infection were detected using real-time quantitative PCR and gene sequencing respectively to evaluate the accuracy of real-time quantitative PCR for detecting chlamydiales pneumoniae. Results Taking gene sequencing as a gold standard,the sensitivity of real-time quantitative PCR was 100. 0%; the specificity was 93. 1%; the positive predictive value was 80. 8%; the negative predictive value was 100. 0%,and the accuracy was 94. 6%. Conclusion Real-time quantitative PCR has high reliability and is suitable for rapid diagnosis of related diseases infected by chlamydiales pneumoniae.

关 键 词：实时荧光定量PCR 肺炎衣原体 

分 类 号：R563.1[医药卫生—呼吸系统]