急性亚硝酸盐胁迫对红耳龟和中华条颈龟幼体抗氧化能力的比较研究  

A comparative study of anti-oxidative ability between Trachemys scripta elegans and Mauremys sinensis under acute nitrite stress

作　　者：裘正元 洪美玲[1] 张豪[1] 陈鹏[1] 史海涛[1,2] 

机构地区：[1]海南师范大学生命科学学院,海口571158 [2]中国科学院成都生物研究所,成都610041

出　　处：《生态科学》2015年第4期99-104,共6页Ecological Science

基　　金：国家自然科学基金项目(31360642;31372228);教育部科技重点项目(211145);省级创新项目(2013116580)

摘　　要：为了比较红耳龟(Trachemys scripta elegans)与本地龟抗氧化损伤的能力,选取体重为5?1 g的红耳龟(n=18)和中华条颈龟(Mauremys sinensis)(n=18)幼体暴露于3个亚硝酸盐浓度组(0、50、500 mg?L–1),进行24 h急性胁迫实验。结果表明:(1)随着亚硝酸盐浓度的升高,红耳龟肝组织中积累的亚硝酸盐量显著低于中华条颈龟(p<0.05),肠组织中的积累量则相反,这说明红耳龟对亚硝酸盐的代谢及解毒能力要强于中华条颈龟;(2)两种龟肝组织和肠组织中超氧化物歧化酶(SOD)活力显著低于对照组(p<0.05),且同一浓度组内红耳龟SOD活力略高于中华条颈龟,但差异不显著;两种龟肝组织和肠组织中丙二醛(MDA)含量随亚硝酸盐浓度的升高而显著升高,相比于红耳龟而言,中华条颈龟的上升趋势更加显著,说明亚硝酸盐浓度的升高对龟体产生了氧化性损伤且中华条颈龟的损伤程度要高于红耳龟;(3)两种龟红细胞微核率随亚硝酸盐浓度升高而升高,且高浓度组中中华条颈龟略高于红耳龟。综上所述,红耳龟幼体在亚硝酸盐胁迫下,其机体的氧化损伤程度要弱于中华条颈龟。In order to compare the ability of stress resistance of environmental pollutants between Trachemys scripta elegans（n=18） and the native Mauremys sinensis（n=18） juvenile, an acute stress of three nitrite concentration groups（0, 50, 500 mg·L^-1） for 24 h was selected. Results showed that：（1） with the increasing of the nitrite concentration, the nitrite accumulated in the liver of T. s. elegans was significantly lower than that in M. sinensis（p〈 0.05）. In contrary, the nitrite accumulated in T. s. elegans was significantly higher than M. sinensis in intestine, which showed that T. s. elegans had a better ability of nitrite metabolism and detoxification than M. sinensis.（2） The Superoxide dismutase（SOD） activity in the liver and intestine of these two turtles in the nitrite treatment groups were significantly lower than the control group（p〈0.05）, and the concentration of the same group of T. s. elegans was a little higher than M. sinensis, but no significant difference existed. However, the malonic dialdehyde（MDA） content in the liver and intestine of these two turtles increased significantly with the rising of nitrite concentration. Compared with T. s. elegans, the MDA of M. sinensis raised more significantly, which indicated a more serious oxidative damage of M. sinensis than T. s. elegans.（3）The erythrocytes micronucleus rates of these two turtles raised with the nitrite concentration, although M. sinensis was a little higher, no significant difference existed. In sum, T. s. elegans juvenile had a slightly stronger anti-oxidative ability than M. sinensis juvenile under nitrite stress.

关 键 词：亚硝酸盐胁迫 微核率 抗氧化酶 红耳龟 中华条颈龟 

分 类 号：Q955[生物学—动物学]