荧光PCR方法快速诊断临产妇及初生儿分泌物标本中的B族溶血性链球菌  

Fluorescence PCR method in rapid diagnosis of group B streptococcus of maternal and neonatal secretion samples

作　　者：黄勇[1] 卢彩玲[2] 应小明[1] 叶辉[1] 闫李侠[3] 杨心宇[2] 陈晓璐[2] 蔡俏[1] 赵佳佳[1] 高未 

机构地区：[1]浙江省台州市第一人民医院新生儿科,台州318020 [2]浙江省台州市第一人民医院妇产科,台州318020 [3]浙江省台州市第一人民医院检验科,台州318020

出　　处：《临床医学》2015年第9期5-7,共3页Clinical Medicine

基　　金：台州市黄岩区课题[2012038(15)]

摘　　要：目的通过实时荧光定量PCR方法对妊娠35～37周孕妇生殖道及直肠分泌物样本及相同孕妇分娩婴儿中的B族溶血性链球菌DNA进行检测，用于临床B族溶血性链球菌感染的辅助诊断。方法选取孕35～37周早产、分娩前胎膜破裂的孕妇43例，同时采集阴道下1／3分泌物及直肠分泌物作为检测标本。对标本进行B族链球菌核酸检测，同时在分娩时采集28株相同孕妇分娩婴儿咽喉部分泌物进行B族链球菌核酸检测。分别观察孕妇及胎膜早破、早产婴儿B族溶血性链球菌感染发生情况。结果7l株I临产妇及初生儿采样标本中，检测到13株感染携带者标本。结论实时荧光定量PCR方法作为临床检测B族溶血性链球菌的一种检测方法，具有检测快速、特异性强的特点。Objective By real-time fluorescence quantitative PCR method for 35 - 37 weeks of pregnancy in pregnant women with genital tract and rectal secretions samples of group B hemolytic streptococcus （GBS） DNA, can be used for the auxiliary di- agnosis of group B streptococcal infection. Methods Forty-three pregnant women with rupture of membranes, 35 to 37 weeks of pregnancy premature were selected, and their vaginal 1/3 secretion and rectal secretions as specimens were collected. Group B Streptococcus on specimen nucleic acid detection, at the same time, nucleic acid detection in 28 cases of baby throat secretions of group B streptococcus. The group B hemolytic streptococcus infection rate were observed in pregnant women and premature rupture of membranes, premature infant. Results Among the 71 strains of parturient and newborn sampling specimens, 13 in- fectedstrains were detected. Conclusion Real time fluorescent quantitative PCR method is a means of clinical detection of GBS, and has quick detection, specificity.

关 键 词：实时荧光定量PCR B族溶血性链球菌 新生儿 

分 类 号：R714.7[医药卫生—妇产科学]