抗CCP抗体,AKA与RF联合检测在类风湿关节炎诊断中的临床意义  

Clinical Significance of Anti-CCP Antibody and Anti-AKA Antibodv and Rheumatoid Factor in Rheumatoid Arthritis

作　　者：赵辉[1] 

机构地区：[1]延安大学附属医院,陕西延安716000

出　　处：《现代检验医学杂志》2015年第5期159-161,共3页Journal of Modern Laboratory Medicine

摘　　要：目的 评价抗CCP抗体,AKA与RF联合检测在类风湿关节炎（RA）诊断中的临床意义.方法 对80例RA患者、103例非RA患者和30例健康对照者血清标本分别采用ELISA法、间接免疫荧光法、散射比浊法检测抗CCP抗体,AKA和RF.结果 ①抗CCP抗体,AKA和RF在非RA组中的阳性检出率分别为2.9％,1.9％和22.3％,RA组阳性检出率分别为71.3％,46.3％和80％,两组间比较差异均具有统计学意义（χ2 =33.4,17.7,24.1,P＜0.05）;②单独检测时,抗CCP抗体,AKA和RF敏感度分别为71.3％,46.3％和80％,特异度分别为96.2％,98.5％和80.5％.联合检测时,抗CCP＋ RF对RA的敏感度最高（48.8％）,两两联合或三者联合检测的特异度显著提高（98.5％～100％）.结论 抗CCP抗体,AKA和RF联合检测可以提高RA早期诊断的准确率,降低误诊率及漏诊率.Objective To evaluate the clinical significance of the combined detection of anti-cyclic citrullinated peptide （Anti- CCP） antibody ,anti-keration antibody （AKA） and rheumatoid factor （RF） for the diagnosis of rheumatoid arthritis （RA） in primary hospital. Methods Anti-CCP antibody was detected by using ELISA and AKA was detected by the indirect im- munofluorescence method and RF was detected by scattering turbidimetry in 80 patients with RA, 103 patients with non-RA rheumatic disease with joint discomfort and 30 healthy subjects as control. Results ①The positive rate of anti-CCP,anti- MCV and RF was 71.3% ,46.3% and 80% respectively in patients with RA,and 2.9% ,1.9% ,22.3% respectively in pa- tients with non-RA, that was significantly higher than those patients with non-RA （χ2 = 33.4,17.7,24. 1 ,P〈0.05）. ②The sensitivity and specificity of anti-CCP antibody was 71.3% and 96.2%, that of AKA was 46.3%, 98.5%, that of RF was 80 % ,80.5 % respectively. The combined detection of anti-CCP antibody and RF had the highest sensitivity （48.8 %）. The combined detection of two or three antibodies had significantly higher specificity （98.5 %- 100%）. Conclusion The com- bined detection of anti-CCP antibody,AKA and RF can significantly improve the diagnostic efficiency and reduce the misdi- agnosis rate and the omission diagnose rate.

关 键 词：抗环瓜氨酸肽抗体 抗角蛋白抗体 类风湿因子 类风湿关节炎 联合检测 

分 类 号：R593.22[医药卫生—内科学] R392.11[医药卫生—临床医学]