小柴胡汤对肝郁脾虚型FD大鼠Ghrelin和胃排空的影响  

Effect of Xiaochaihu decoction on expression of Ghrelin and gastrointestinal motility in functional dyspepsia rats with syndrome of liver stagnation and spleen deficiency

作　　者：钟欢[1] 郁保生[2] 邹旭峰[2] 戴维[2] 刘未艾[1] 葛君芸[1] 刘密[1] 常小荣[1] 

机构地区：[1]湖南中医药大学针灸推拿学院,湖南省长沙市410007 [2]湖南中医药大学中医学院,湖南省长沙市410007

出　　处：《世界华人消化杂志》2015年第28期4542-4547,共6页World Chinese Journal of Digestology

基　　金：长沙市科技计划项目;No.K1308046-31~~

摘　　要：目的:观察小柴胡汤对肝郁脾虚型功能性消化不良(functional dyspepsia,FD)模型大鼠脑、脊髓、胃肠组织中胃促生长素(Ghrelin)及胃排空的影响,探讨小柴胡汤治疗FD的可能机制.方法:60只清洁级SD大鼠随机分为空白组、模型组、小柴胡汤组、逍遥散组和多潘立酮组5组,每组12只,分组治疗14 d后行胃排空实验并计算胃残留率,Western blot法检测下丘脑、脊髓、胃窦、结肠组织Ghrelin蛋白的表达.结果:小柴胡汤组、多潘立酮组胃残留率明显低于模型组(P<0.01);小柴胡汤组、逍遥散组、多潘立酮组下丘脑、脊髓、胃Ghrelin蛋白表达显著高于模型组;3组间比较小柴胡汤组效果明显优于逍遥散组(P<0.01),但与多潘立酮组相比无显著差异.结论:小柴胡汤组能和多潘立酮组一样通过增加组织中Ghrelin蛋白的表达,从而达到促进胃排空的作用,这可能是小柴胡汤治疗肝郁脾虚型消化不良的可能机制之一.AIM：To observe the influence of Xiaochaihu decoction on the expression of Ghrelin and gastrointestinal motility in functional dyspepsia（FD） rats with syndrome of liver stagnation and spleen deficiency and explore the possible mechanism for Xiaochaihu decoction to treat FD.METHODS：Sixty SD rats were randomly divided into five groups：a blank group,a model group,a Xiaochaihu decoction group,a Xiaoyaosan group and a domperidone group,with 12 rats in each group.After 14 d of treatment,gastric emptying was measured,and the expression of Ghrelin in the hypothalamus,spinal cord,gastric antrum,and colon was detected by Western blot.RESULTS：The gastric emptying rates in the Xiaochaihu decoction group,Xiaoyaosan group and domperidone group were significantly higher than that of the model group（P〈0.01）,while the expression of Ghrelin was significantly higher than that of the model group（P〈0.01,P〈0.05,P〈0.01）;there were also significant differences between the Xiaoyaosan group and Xiaochaihu group.CONCLUSION：Xiaochaihu decoction,similar to domperidone,could promote the gastric emptying by increasing the expression of Ghrelin in the tissue;this might be one of the mechanisms of action of Xiaochaihu decoction in FD.

关 键 词：小柴胡汤 功能性消化不良 胃促生长素 胃排空 肝郁脾虚 

分 类 号：R285.5[医药卫生—中药学]