五唇兰根部内生细菌的筛选及其促生活性  

Activity Analysis of Growth-promoting Endophytic Bacteria Isolated from the Roots of Phalaenopsis pulcherrima

作　　者：张芳芳[1] 宋希强[1] 丁琼[1] 朱国鹏[1] 

机构地区：[1]海南大学园艺园林学院/热带作物种质资源保护与开发利用教育部重点实验室,海南海口570228

出　　处：《热带生物学报》2015年第3期285-292,共8页Journal of Tropical Biology

基　　金：海南省自然科学基金[海南濒危兰科植物五唇兰种群动态监测的保育研究(312063)]资助;海南省科技成果示范推广专项[朵丽蝴蝶兰高效增产栽培技术示范与推广(CGSF20120001-02)]

摘　　要：以野生五唇兰植株根部中分离出的15株内生细菌为研究对象,将其接入五唇兰组培苗中进行共培养,研究其对组培苗的促生影响并分析其固氮、溶磷和产IAA能力等促生活性。结果表明,15株内生细菌中有11株对五唇兰组培苗有促生作用,其中,Paenibacillus graminis(TW2-1)对五唇兰组培苗有显著的促生影响,促生效果最佳。15株内生细菌中,9株具潜在固氮能力;10株具产IAA的能力,其中Bacillus cereus(TW1-4)产IAA能力最强;7株具溶解无机磷能力,Bacillus megaterium(TW1-1)溶磷量最高,内生细菌的溶磷能力与其产酸能力呈正相关。Endophytic bacteria of orchid plants play an important role in orchid life cycle. An attempt was made to inoculate 15 endophytic bacteria isolated from the root of wild plants Phalaenopsis pulcherrima into Phalaenopsis pulcherrima plantlets in vitro for co-culture to analyze their abilities of nitrogen fixation,phosphate solubilization and IAA production. Of the 15 endophytic bacteria,11 strains were found to have a growth-promoting effect on P. pulcherrima plantlets,of which the strain Paenibacillus graminis produced best growth-promoting effect; 9 strains had a potential ability of nitrogen fixation; 10 strains were able to produce IAA,of which the strain Bacillus cereus produced the highest amount of IAA; 7 strains was able to dissolve phosphorus,of which Bacillus megaterium dissolved the highest amount of phosphorus. In addition,endophytic bacteria had a positive correlation between the ability of phosphate-solubilization and the ability of IAA production.

关 键 词：五唇兰 内生细菌 植物促生活性 

分 类 号：S182[农业科学—农业基础科学]