模拟水淹对水杉苗木生长与生理生化特性的影响  

Effects of simulated waterlogging on growth,physiological and biochemical characteristics of Metasequoia glyptostroboides seedlings

作　　者：白林利 韩文娇[1] 李昌晓[1] 

机构地区：[1]西南大学生命科学学院,三峡库区生态环境教育部重点实验室,重庆400715

出　　处：《浙江大学学报（农业与生命科学版）》2015年第5期505-515,共11页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：重庆市基础与前沿研究计划重点项目(CSTC2013JJB00004);中央高校基本科研业务费专项资金(XDJK2013A011);国家林业公益性行业科研专项(201004039);留学回国人员科研启动基金(教外司留[2010]1561号)

摘　　要：通过对在不同土壤水分处理下水杉(Metasequoia glyptostroboides)保护酶系[超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)、抗坏血酸过氧化物酶(ascorbate peroxidase,ASP)、过氧化氢酶(catalase,CAT)]活性及渗透调节物质(可溶性蛋白质、游离脯氨酸)和细胞膜脂过氧化产物[丙二醛(malondialdehyde,MDA)]含量以及光合特性、生物量的测定,探讨水杉对三峡库区水位变化的响应特性和耐水淹适应能力。模拟三峡库区消落带土壤水分变化格局,以二年生水杉苗木为试验材料,设置对照组(control,CK)、半淹组(half-submersion,HS)和全淹组(full-submersion,FS)3个处理。结果表明:在水淹胁迫下,SOD、POD、ASP、CAT活性以及游离脯氨酸含量均升高。在水淹胁迫下,水杉叶片MDA含量与CK相比差异不显著,无统计学意义。HS净光合速率(net photosynthetic rate,Pn)显著高于CK;FS的根冠比显著高于CK,但与HS相比差异不显著,无统计学意义。全淹植株呈叶芽形式,水淹植株存活率均达100%。在淹水期间,由于抗氧化酶、渗透调节物质、光系统Ⅱ的积极响应,水杉表现出极强的水分适应能力。因此,可以考虑将水杉列为三峡库区消落带植被构建的候选树种之一。The disruption of natural flow regimes in river systems poses many challenges to riparian ecosystems and their native species.The construction of the Three Gorges Dam has altered the flow regimes of the upper Yangtze River and created a riparian zone with a vertical gap of 30 m.Because of the anti-seasonal change of the water level caused by annual water regulation,plants grown on the riparian zone of the Three Gorges Reservoir Area（TGRA）may suffer from submergence,and often display dynamic change characteristics.Such water level change is likely to disturb the normal ecophysiological rhythm of the native tree species of the riparian zone.These hydrological changes highlight the importance of screening suitable tree species for reforestation in the TGRA and similar environments.Thus,the native tree species Metasequoia glyptostroboides,will most likely to experience continuous submergence or inundation.Current research on M.glyptostroboides seedlings is more focused ongenetic structure,membrane composition,chemical property,growth and photosynthesis,and the like.However,the eco-physiological implications of submersion on M.glyptostroboides seedlings are not well known,especially under the condition of full-submersion.The aim of this study was to investigate the responding characteristics of the M.glyptostroboides seedlings to the water level change in the TGRA,and provide theoretical basis for species selection for revegetation in the riparian zone of the TGRA.Measured indexes included protective enzymes such as superoxide dismutase（SOD）,peroxidase（POD）,ascorbate peroxidase（ASP）and catalase（CAT）,osmotic adjustment substances such as soluble protein and free proline,and membrane lipid peroxidation such as malondialdehyde（MDA）,as well as photosynthetic characteristics and biomass accumulation of the two-year old M.glyptostroboides seedlings to submergence,upon mimicking the water level change in the riparian zone of the TGRA.Based on soil moisture change pattern in the TGRA,water treatments i

关 键 词：水杉 水分胁迫 生长 生理生化 

分 类 号：Q945.78[生物学—植物学] S718.43[农业科学—林学]