毛细管电泳法测定螺内酯片剂中螺内酯的含量  

Content of spironolactone in the spironolactone tablet determined by capillary electrophoresis

作　　者：于海霞[1] 李露[2] 马慧芬[2] 张红芬[2] 陈安家[2] 

机构地区：[1]山西医科大学细胞生理学省部共建教育部重点实验室,太原030001 [2]山西医科大学药学院

出　　处：《山西医科大学学报》2015年第10期995-998,共4页Journal of Shanxi Medical University

基　　金：山西医科大学创新基金资助项目(01201305)

摘　　要：目的建立可测定螺内酯片剂中螺内酯含量的毛细管电泳法。方法采用BECKMAN毛细管电泳仪;未涂层毛细管(75μm×57 cm,有效长度10.2 cm);二极管阵列检测器(DAD);以含0.004 mmol/L的十六烷基三甲基溴化铵(CTAB)和20%乙腈的p H6.5磷酸盐缓冲液为背景电解质溶液,浓度为0.020 mol/L;分离电压20 k V;检测波长244 nm。结果螺内酯在1.62-119.9μg/ml浓度范围内线性关系良好,线性方程为Y=877.7X+4 238(r2=0.991 6);回收率91.2%-106.6%;精密度、稳定性、重复性均较好。结论毛细管电泳法测定螺内酯片中螺内酯含量,方法简便、快速、专属性强,可用于药物制剂中螺内酯含量的测定。Objective To establish a method for determining the content of spironolactone in spironolactone tablet by capillary electrophoresis. Methods Capillary electrophoresis apparatus with diode array detector was adopted. The uncoated fused silica capillary（ 75μm × 57 cm,effective length of 10. 2 cm） was available. The p H 6. 5 phosphate buffer solution containing 20% acetonitrile and 0. 004 mmol / L cetyl trimethyl ammonium bromide（ CTAB） were chosen as the background electrolyte,and the concentration of background electrolyte was 0. 020 mol / L. The analysis voltage was set at 20 k V and the detection wavelength was set at 244 nm. Results There was a good linear relationship between the peak area and the concentration of spironolactone in the range of 1. 62- 119. 9 μg / ml,and the linear regression equation was Y = 877. 7X ＋ 4 238（ r2= 0. 991 6）. The recovery rate was 91. 2%- 106. 6%. The precision,stability,repeatability of the method were good. Conclusion The developed method is simple,rapid and specific,which can be used for the determination of spironolactone in pharmaceutical preparation.

关 键 词：螺内酯 毛细管电泳法 含量测定 

分 类 号：R927.2[医药卫生—药学]