白色念珠菌ALS基因家族在生物膜形成过程中的差异表达  

Differential Expression of Candida Albicans ALS Gene Family During Biofilm Formation In Vitro

作　　者：何淼[1,2] 宋光泰[2] 边专[1] 

机构地区：[1]武汉大学口腔医学院.口腔基础医学省部共建国家重点实验室培育基地和口腔生物医学教育部重点实验室 [2]武汉大学口腔医院儿童口腔科,湖北武汉430079

出　　处：《口腔医学研究》2015年第10期957-960,共4页Journal of Oral Science Research

基　　金：国家自然科学基金资助(编号:81300870)

摘　　要：目的:研究白色念珠菌凝集素样粘附素(agglutinin-like sequence,ALS)基因家族成员在生物膜形成过程中的表达。方法:构建白色念珠菌标准株ATCC 10231和ATCC 90028体外生物膜模型,在生物膜形成的早期、中期和成熟期分别提取总RNA,应用半定量RT-PCR检测8个ALS基因的表达情况。结果:在生物膜形成的整个过程中,ALS1、ALS2、ALS3和ALS5持续高表达,是主要表达的粘附素基因,ALS6、ALS7、ALS9,尤其是ALS4表达较弱。ALS1、ALS2和ALS3在生物膜形成中期比在早期表达显著上调,但在成熟期表达下降。ALS基因家族成员的表达模式在菌株间无明显差异。结论:ALS1、ALS2、ALS3和ALS5在白色念珠菌体外生物膜形成过程中优势表达,对这些ALS基因的靶向抑制将为控制白色念珠菌生物膜相关疾病提供新的思路。Objective：To investigate the differential expression of Candida albicans（C.albicans）agglutinin-like sequence（ALS）gene family during biofilm formation on biomaterial surfaces in vitro.Methods：The biofilm model of C.albicans standard strain ATCC 10231 and ATCC 90028 was established in vitro.Expression of the eight members in C.albicans ALS gene family was studied by semi-quantitative reverse transcription-polymerase chain reaction（RT-PCR）of total RNA from the in vitro biofilm samples on biomaterial surfaces at three distinct developmental phases.Results：ALS1,ALS2,ALS3 and ALS5transcripts were detected predominantly and consistently over time during biofilm formation.The expression of ALS6,ALS7 and ALS9,especially ALS4,was weak.Transcript amounts of ALS1,ALS2 and ALS3were up-regulated significantly in intermediate phase and then downregulated in maturation phase.Strain variability of ALS gene expression was not observed in this study.Conclusion：ALS1,ALS2,ALS3 and ALS5are predominantly expressed during biofilm formation in vitro.Targeted inhibition of these ALS genes provides potential therapeutic targets to control C.albicans biofilm-associated diseases.

关 键 词：白色念珠菌 ALS基因家族 生物膜 

分 类 号：R379.4[医药卫生—病原生物学]