宽体沙鳅精子生物学特性及水体和pH对其活力的影响  

Main Biological Characteristics and Influences of Different Aqueous Solution, and p H on Sinibotia reevesae Sperm

作　　者：周露[1] 陈瑜[1] 王永明[1] 

机构地区：[1]内江师范学院生命科学学院,四川内江641199

出　　处：《水产学杂志》2015年第5期8-11,共4页Chinese Journal of Fisheries

基　　金：内江师范学院2014年度大学生科研项目(14NSD-39)

摘　　要：研究宽体沙鳅Sinibotia reevesae精子的形态、密度、p H及水体（沱江-内江段江水、曝气井水、曝气蒸馏水和曝气自来水）、p H（5.5、6.0、6.5、7.0、7.5、8.0、8.5和9.0）对宽体沙鳅精子活力的影响。结果显示：宽体沙鳅精子由头部、中片及尾部三部分构成,头部短径（2.181±0.148）μm,长径（2.525±0.197）μm,中片甚短,光镜下难辨清,尾部长（31.905±2.869）μm;精子密度为（1.443±0.292）×1010ind/m L;p H6.7~7.0;精子在曝气井水中活力最高,其快速运动时间、有效运动时间及寿命分别为（8.82±0.97）s、（15.13±1.18）s和（35.71±2.90）s;精子在p H7.5时活力最强,精子的快速运动时间、有效运动时间及寿命分别为（11.91±1.66）s、（14.87±1.94）s和（38.34±1.67）s。可根据精子形态、密度和p H初步判断精子活力;宽体沙鳅精子激活介质宜采用p H7.5的曝气井水。The shape, density, p H of sperm and the effects of different aqueous solution（water collected in section of Tuojiang-Neijiang river, exposed well water, exposed distalled water, and exposed tape water）, and p H（5.5、6.0、6.5、7.0、7.5、8.0、8.5 and 9.0）on sperm motility were investigated in Sinibotia reevesae. The results showed that the sperm had a head（2.181±0.148）μm in width and（2.525±0.197）μm in length, a mid-piece, hard to be seen under a microscope and a long flag about（31.905±2.869）μm in length,with density of（1.443 ± 0.292）×1010 ind/m L and p H of 6.7~7.0. The sperm showed the maximal motility, movement time [（8.82±0.97）s], effective movement time [（15.13±1.18）s] and sperm life time [（35.71±2.90）s] in the well water. Furthermore, the fast movement time [（11.91±1.66）s], effect movement time [（14.87±1.94）s] and long sperm life time [（38.34±1.67）s] were observed in the sperm in the distilled exposed to air with p H of 7.5. It is suggested that the main characteristics of sperm can be regarded as evidences for selecting superior sperm and that the well water with p H 7.5 be the optimal medium for activation of S. reevesae sperm.

关 键 词：宽体沙鳅 精子形态 精子活力 精子寿命 

分 类 号：Q492[生物学—生理学]