罗丹明类小分子荧光探针对水中Hg^(2+)检测的比较研究  

Comparison of detection for Hg^(2+) in water by rhodamine fluorescence probes

作　　者：吕向菲[1,2] 李婷[1] 霍霄楠 胡南江[3] 闫蕾[4] 李国荣[1] Ermelinda Bloise 

机构地区：[1]陕西科技大学资源与环境学院,陕西西安710021 [2]Department of Engineering for Innovation,University of Salento,italylecce73100 [3]陕西科技大学轻工与能源学院,陕西西安710021 [4]国家知识产权局专利局专利审查协作北京中心材料工程发明审查部,北京100096

出　　处：《陕西科技大学学报（自然科学版）》2015年第5期34-39,共6页Journal of Shaanxi University of Science & Technology

基　　金：陕西省科技厅自然科学基金项目(2013JQ2026);国家级大学生创新创业训练计划项目(201310708013);陕西科技大学学术骨干培育计划项目(XSG(4)012)

摘　　要：将罗丹明B(RhB)及罗丹明6G(Rh6G)应用于水中Hg2+的荧光猝灭法测定.在KI存在时,Hg2+可与罗丹明类小分子及KI形成多元离子缔合物,故在水中可直接利用体系的荧光发射峰强度测定Hg2+.通过比较两种罗丹明类荧光探针的检测特性发现,两种罗丹明类小分子都是荧光检测特性较好的猝灭型探针,其中罗丹明6G由于其分子结构稳定性大,故灵敏性、线性范围和选择性均高于罗丹明B.The rhodamine B and rhodamine 6G were applied to the determine of Hg2＋ in water by quenching‐fluorescence .After the addition of KI ,Hg2＋ could produce multi‐ionic associat‐ed substance with rhodamine probes ,which was used to detect the Hg2＋ in water solution through the intensity of fluorescence emission peak in system .By comparision of the detec‐tion performance based two probes ,the results indicated that two rhodamine moleculers were quenching fluorescence probes and the rhodamine 6G showed higher sensitivity ,linearity and selectivity than rhodamine B ,due to its steady structure .

关 键 词：荧光猝灭 罗丹明B 罗丹明6G 

分 类 号：O657.3[理学—分析化学]