银杏叶提取物对大鼠非酒精性脂肪肝炎肝脏保护作用的研究  

Protective effect of ginkgo biloba extract on liver nonalcoholic steatohepatitis rats

作　　者：龚毅[1] 范伟[2] 张莹[3] 侯炬 郭江福[2] 柳杨[2] 

机构地区：[1]遵义医学院研究生院,贵州遵义563003 [2]贵州省人民医院急诊外科,贵阳550002 [3]贵州省人民医院肝胆外科,贵阳550002

出　　处：《重庆医学》2015年第30期4190-4192,共3页Chongqing medicine

基　　金：贵州省省长基金[黔科教办(2007)04];贵州省科技厅基金[黔科合J(2008)2301];贵州省科技厅专项基金[黔科合J(2008)-2302号];贵州省优秀青年科技人才基金[黔科合人字(2011)29号]

摘　　要：目的观察银杏叶提取物对非酒精性脂肪肝炎大鼠Toll样受体4(TLR4)、肿瘤坏死因子α(TNF-α)等表达的影响。方法雄性SD大鼠分为3组。对照组正常饮食,模型组、治疗组给予高脂饲料喂养16周诱发脂肪性肝炎后分别以生理盐水、银杏叶提取物液10mL/kg灌胃,每日1次,共8周。处死大鼠,分别进行如下检测:(1)观察肝脏病理变化;(2)检测TLR4表达水平;(3)检测血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)水平及血清TNF-α水平。结果 (1)治疗组和模型组均有不同程度的肝细胞脂肪变性,且治疗组轻于模型组。(2)模型组、治疗组TLR4mRNA及蛋白、TNF-α表达高于对照组,模型组又高于治疗组,差异有统计学意义。对照组血清ALT、AST明显低于模型组及治疗组,治疗组又低于模型组,差异有统计学意义(P<0.05)。结论银杏叶提取物对非酒精性脂肪肝炎大鼠有较好的肝脏保护作用,其可能机制与降低TLR4的表达、抑制炎性反应有关。Objective To observe the effect of ginkgo biloba extract on the expression of TLR4 and TNF‐αin rat nonalcohol‐ic steatohepatitis .Methods SD rats were randomly divided into three groups ：control group （normal diet） ,rats in the model group and the treatment group was given high fat diet to induce steatohepatitis for 16 weeks ,respectively .Rats in model group were trea‐ted with normal saline ,rats in treatment group was fed ginkgo biloba extract （10 mL/kg per day ,for eight weeks） .Then rats were sacrificed ,the following tests were performed：（1）The pathological changes in liver;（2）the expression level of TLR4 ;（3）serum ALT ,AST levels and serum TNF‐α levels .Results （1）Both treatment group and model group have different levels of hepatic steatosis ,and it was lighter in the treatment group .（2）TLR4 mRNA and its protein and TNF‐α expression in model group and treatment group were higher than that of the control group ,and the model group was higher than the treatment group ,the differ‐ence was statistically significant .Serum ALT and AST was significantly lower in control group than the model group and the treat‐ment group ,the treatment group was lower than model group ,the difference was statistically significant （P＆lt;0 .05） .Conclusion Ginkgo biloba extract has good protection effect on nonalcoholic steatohepatitis in rats ,and the possible mechanism may be the re‐duction of the expression of TLR4 and the inhibition of inflammatory reaction

关 键 词：银杏叶提取物 脂肪肝 TOLL样受体 肿瘤坏死因子Α 

分 类 号：R285.5[医药卫生—中药学]