微藻油脂含量不同测定方法的比较研究  

Comparison of detection methods of total lipid content in microalgae

作　　者：彭悦[1] 刘玉莹[1] 祁艳霞[2] 胡玉才[1] 付晚涛[3] 

机构地区：[1]大连海洋大学理学院,辽宁大连116023 [2]大连海洋大学食品科学与工程学院,辽宁大连116023 [3]大连海洋大学海洋科技与环境学院,辽宁大连116023

出　　处：《大连海洋大学学报》2015年第5期455-460,共6页Journal of Dalian Ocean University

基　　金：辽宁省海洋与渔业厅计划项目(201215)

摘　　要：为了对微藻油脂含量不同测定方法进行比较,以球等鞭金藻8701 Isochrysis galbana Parke 8701和小新月菱形藻Nitzschia closterium f.minutissima两种微藻为研究对象,采用溶剂提取法和苏丹黑B染色法对其油脂含量进行测定,建立了两种微藻油脂含量与苏丹黑染色后吸光度A645 nm的线性回归方程,并应用于微藻油脂积累培养过程的快速测定,利用气相色谱法对两种微藻脂肪酸组成进行分析。结果表明:当提取溶剂为二氯甲烷和甲醇的混合溶剂(二者的体积比为1∶2)时,油脂提取效果较好,得到球等鞭金藻8701和小新月菱形藻的油脂含量分别为22.99%和16.89%;采用苏丹黑B染色法测定的油脂含量基本和生物量大体一致,是一种可行的估测油脂含量的方法;气相色谱检测结果显示,两种微藻具有明显不同的脂肪酸组成特征,球等鞭金藻8701的饱和脂肪酸含量(55.02%)较小新月菱形藻(51.39%)高。研究表明,从两种微藻的油脂含量和脂肪酸组成来看,球等鞭金藻8701较小新月菱形藻更适用于作为生物柴油的原料。The total lipid contents were analyzed by methods of organic solvents and Sudan black B staining in two species of microalgae, Isochrysis galbana Parke 8701 and Nitzschia closterium f. minutissima. linear regression e-quations were established between lipid contents and absorbance at a wavelength of 645 nm. It was found that mi-croalgae cells were stained with Sudan black B, and applied to rapid determination of the microalgae oil accumula-tion rapidly. Fatty acid compositions were also analyzed by gas chromatography （ GC） . The extraction of solvent di-chloromethane/methanol （ volume ratio =1：2 ） revealed that there was total lipid content of 22 . 99% in Isochrysis galbana Parke 8701, and 16. 89% in Nitzschia closterium f. minutissima. The total lipid was consistent with the bi-omass by Sudan black B staining method, indicating that Sudan black B staining method is a feasible method to es-timate lipid content. There were significant differences in fatty acid compositions determined by GC in the two mi-croalgae, with higher saturated fatty acid in Isochrysis galbana Parke 8701 （55. 02%） than that in Nitzschia closte-rium f. minutissima（51. 39%）. The findings indicate that Isochrysis galbana Parke 8701 is more suitable as a feed-stock for biodiesel in terms of total lipid content and fatty acid composition.

关 键 词：微藻 油脂 苏丹黑B染色法 溶剂提取法 脂肪酸 

分 类 号：Q949.2[生物学—植物学]