烟曲霉YSITB Ⅰ菌株筛选及其降解木质纤维中木质素研究  

Screening of Aspergillus Fumigatus YSITBI and Its Lignin Degradation Ability

作　　者：袁俊超[1] 吕宝[1] 屈敦妮 董淑君[1] 徐超[1] 郭诗仪 熊燕飞[1] 

机构地区：[1]武汉理工大学化学化工与生命科学学院,武汉430070

出　　处：《湖北农业科学》2015年第17期4164-4167,4172,共5页Hubei Agricultural Sciences

基　　金：武汉理工大学国家级创新创业训练项目(20141049714007)

摘　　要：天牛幼虫肠道菌群具有分解木质纤维的功能。本研究以木质纤维为唯一碳源,从天牛幼虫肠道中筛选出三株对木纤维具有降解能力的菌株,对其中的烟曲霉YSITB I菌株降解木纤维中木质素能力及降解条件进行研究,并对三种菌株降解木纤维中木质素的协同作用进行了初步观察。结果显示,烟曲霉YSITB I菌株具有较好的木质素降解作用,在30℃、pH值6.8条件下,对构树木质纤维中木质素的降解率可达56.9%;枯草芽孢杆菌YSITBII和烟曲霉YSITB I协同作用可提高木质素降解效率至64.8%;烟曲霉YSITB I 15d降解木纤维效率高于40%。烟曲霉对木质纤维的降解作用达到甚至高于模式菌-黄包原毛平革菌,降解木纤维程度较高,用于木质纤维脱木质素或秸秆降解沤肥具有很好的应用前景。The intestinal flora of longicorn larva could decompose lignocellulose. Using lignocellulose as the unique carbon source, three strains with the ablity of decomposing lignin were isolated from intestine of the larva. The degradation ability and degradation conditions of lignin in lignocellulose of Aspergillus fumigatus YSITBI as well as the synergistic action of the three strains were studied. The results showed that A . fumigatus YSITBI was good at decomposing lignin, under the condi-tions of 30 ℃, pH 6.8, the degradability of lignin in lignocellulose reached to 56.9%. The cooperation of Bacillus subtilis YSITBII and A . fumigatus YSITBI could improve the degradability of lignin to 64.8%. The degradability of A . fumigatus YSITBI achieved or was even higher than the model bacteria Phanerochaete chrysosporium, thus had a very good prospect in decomposing the lignin from lignocellulose or decomposing straws for fertilizer.

关 键 词：木质素 天牛幼虫 温度 协同性 烟曲霉菌 沤肥 

分 类 号：S182[农业科学—农业基础科学]