真菌SF-21483产生的活性物质分离纯化及初步确认  

Isolation, Purification and Identification of Agro-active Metabolites from Unidentified Fungus Strain SF-21483

作　　者：张亚妮[1] 万中义[1] 张志刚[1] 王燕[1] 杨自文[1] 王开梅[1] 

机构地区：[1]湖北省生物农药工程研究中心/湖北省农业科技创新中心生物农药分中心,武汉430064

出　　处：《湖北农业科学》2015年第17期4188-4191,共4页Hubei Agricultural Sciences

基　　金：公益性行业(农业)科研专项项目(200903052);湖北省农业科技创新中心创新团队项目(2007-620-001-08)

摘　　要：从武汉地区土壤中分离得到一株真菌菌株SF-21483,其发酵液具有较强的抑制真菌的作用。本研究以真菌SF-21483发酵提取物中的活性代谢产物为研究对象,应用高效制备液相色谱经过2次分离纯化,得到了5个化合物。根据紫外吸收光谱、分子离子峰、物理性状及活性情况等确定组分4、5、7、9、10与冬青生菌素(Ilicicolin)F、E、D、C、H较为相似,对植物病原菌生物活性测试表明,该类组分具有较强的抗菌作用。A strain of fungus was isolated from one soil sample collected at Wuhan area, and its fermentation inhibited the growth of phytophathogenic fungi. The strain’s active metabolites were isolated and purified by preparative HPLC. 5 com-pounds were isolated from the fermentation extract of the SF-21483, and which were identified as Ilicicolin F、E、D、C and H, according to UV absorption spectrum, molecular weight, physical description and activities. Biological activity tests showed that the compounds had good fungicidal activity.

关 键 词：真菌菌株SF-21483 农药活性物质 分离 纯化 冬青生菌素 

分 类 号：S482.2[农业科学—农药学]