巨菌草单宁成分的提取和测定  

Extraction and Content Determination of Tannin in Pennisetum sinese

作　　者：谢焰锋 许林 戢小梅 陈法志 罗梓珊 王湛昌 

机构地区：[1]武汉市林业果树科学研究所,武汉430075

出　　处：《湖北农业科学》2015年第17期4250-4252,共3页Hubei Agricultural Sciences

基　　金：武汉市科技攻关项目(2014021302010449;2013021702010587)

摘　　要：以生长1、2、3个月的巨菌草(Pennisetum sinese)叶片为材料,探讨巨菌草叶片单宁成分的提取方法和含量,单宁提取方法采用正交试验进行优化,单宁含量采用福林酚-比色法进行测定。结果表明,巨菌草单宁最佳提取条件为丙酮体积分数70%,提取温度50℃,提取时间1 h。生长1、2、3个月的巨菌草叶片的单宁含量分别为8.47、10.44和12.59 mg/g,与其他牧草相比处于中间水平,且单宁含量随生长时间的延长而增加,不同生长阶段的单宁含量适合不同的动物采食。The content of total tannins in the leaves of Pennisetum sinese was determined by folin phenol colorimetric method. Effects of concentration of extracting solution, temperature and time on tannins extraction were investigated by orthogonal experiment. The optimum extraction conditions were as follows： the samples were extracted for 1 hour at 50 ℃with 70% acetone as the solvent. The different contents of total tannins in the leaves cultivated for 1,2 or 3 month were 8.47,10.44 and 12.59 mg/g respectively. Compared with other forage grasses, the total tannins in the leaves of P. sinese were in the middle range and increased monthly. Correspondingly, the leaves at different growth stages were suitable for feeding different animals.

关 键 词：巨菌草(Pennisetum sinese) 牧草 单宁 正交试验 

分 类 号：S543.9[农业科学—作物学]