披针叶黄华中2株蠕形分生孢子内生真菌的鉴定  

Identification of Two Helminthosporioid Fungal Endophytes from Thermopsis lanceolata

作　　者：孙大林[1] 赵清梅[1] 余永涛[2] 罗永红[1] 朱海[1] 吴勇[1] 郭浩满 吴秀枝[1] 李明月[1] 

机构地区：[1]北方民族大学生物科学与工程学院,银川750021 [2]宁夏大学农学院,银川750021

出　　处：《湖北农业科学》2015年第18期4473-4478,共6页Hubei Agricultural Sciences

基　　金：北方民族大学"2014大学生创新团队项目"(TDXM-2014-011);宁夏自然科学基金项目(NZ12202);国家自然科学基金项目(31201962)

摘　　要：对披针叶黄华(Thermopsis lanceolata)中分离的编号为SP01、RM02的2株蠕形分生孢子内生真菌进行种属分类鉴定。观察真菌的形态,提取真菌DNA,扩增并测定其ITS、GAPDH序列,构建系统发育树,结合形态特征和遗传进化分析结果,并对其进行种属分类。结果表明,SP01菌株的形态特征与凸脐孢属(Exserohilum)中的E.rostratum最为相似,与E.rostratum的遗传进化关系最为密切;RM02菌株的形态特征与弯孢属(Curvularia)中C.spicifera、C.australiensis最为相似,与C.spicifera的遗传进化关系最为密切。确定披针叶黄华中分离的SP01、RM02分别为E.rostratum和C.spicifera。This study was conducted to confirm the species of two helminthosporioid fungal endophytes, SP01 and RM02,isolated from wild Thermopsis lanceolata. The two isolates were observed under the light microscope respectively. The DNA of the fungi were extracted for amplifying the fungal ITS and GAPDH sequences that were sequenced subsequently. Homology comparison was conducted and NJ phylogenetic trees were established based on the sequences of the fungi. Two fungal endophytes were classified according to the fungal morphology and phylogenetic analysis. The results indicated that SP01 was similar to Exserohilum rostratum. Homology and phylogenetic analyses also showed SP01 to be more closely related to E. rostratum. RM02 was more similar to some fungi of Curvularia genus, such as C. spicifera and C. australiensis. Homology and phylogenetic analyses also showed the isolate to be most closely related to C. spicifera. In addition to above results, the two isolates, SP01 and RM02, were classified as E. rostratum and C. spicifera respectively.

关 键 词：披针叶黄华(Thermopsis lanceolata) 内生真菌 蠕形分生孢子真菌 鉴定 

分 类 号：S432[农业科学—植物病理学]