家蚕黑胸败血病病原菌的分离鉴定  

Isolation and Identification on Pathogens of Silkworm Cepticaemia

作　　者：覃玥[1] 安慧[1] 高丽霞[1] 黄小弟[1] 朱彦瑾[1] 陈岩岩[1] 

机构地区：[1]河池学院化学与生物工程学院,广西宜州546300

出　　处：《湖北农业科学》2015年第18期4529-4531,共3页Hubei Agricultural Sciences

基　　金：广西壮族自治区教育厅科研项目(201010LX480);广西壮族自治区教育厅项目(2014JGA214)

摘　　要：从广西宜州蚕区暴发黑胸败血病的养蚕农户处采集到病蚕样本,分离到1株病原菌,通过形态学观察和回复感染健康5龄家蚕试验及16S rDNA基因序列同源性分析鉴定,结果显示病原菌菌落呈橘黄色,光滑,圆形,边缘整齐,有光泽,湿润有黏性,革兰氏染色呈阳性,该菌形态学符合葡萄球菌属的特征;用菌液穿刺感染健康5龄家蚕10 h后蚕体胸部膨大,痉挛侧倒而死,11 h后胸部变黑呈现黑胸败血病病症;从腹腔接种菌液的小白鼠16 h内全部发病,从发病的家蚕体液和死亡小鼠的心血均能分离到与接种菌株形态一致的细菌,证实该菌为引起家蚕黑胸败血病的病原菌。菌株16S rDNA片段为1 463 bp(Gen Bank ID:1811543),与GenBank数据进行比较,该菌株与松鼠葡萄球菌(Staphylococcus sciuri)同源性为99.9%,鉴定该菌株为松鼠葡萄球菌。A strain of pathogen was isolated from sick silkworm which diagnosed cepticaemia in Yizhou. After morphological observation, reverse infection test on healthy five-year-old silkworm and 16 s rDNA homology analysis, the results showed that the colony were orange, smooth, circular, neat, luster, wet sticky, and with Gram positive, the morphology of the bacterium was in line with characteristics of staphylococcus. Using bacteria liquid to puncture the infected five-year-old healthy silkworm, its body chest enlarged 10 hours later and died with spasm. After 11 hours, its chest gradually turned into the symptom of cepticaemia. All the mice vaccinated the bacteria liquid from its vacuum were attacked of a disease in 16 hours, and a kind of germ with the same form of vaccinated bacteria was isolated both from the body fluid of sick silkworm and the heart blood and liver of the dead mouse which proved the pathogenic germs caused the silkworm cepticaemia. The 16 s rDNA fragment of the strain was 1 463 bp（Gene Bank ID： 1811543）, and its homology with Staphylococcus sciuri was 99.9%. The bacteria germ strain was identified as Staphylococcus sciuri.

关 键 词：家蚕细菌病 黑胸败血病 松鼠葡萄球菌(Staphylococcus sciuri) 16Sr DNA 分子鉴定 

分 类 号：S884.42[农业科学—特种经济动物饲养]