水稻OsCER4基因启动子序列及表达分析  

Promoter Sequence and Expression Analysis of OsCER4 Gene in Rice

作　　者：林晓林[1] 罗苑红 梁丽凤[1] 冯君成[1] 周玲艳[1] 

机构地区：[1]仲恺农业工程学院生命科学学院,广州510225

出　　处：《湖北农业科学》2015年第18期4607-4612,共6页Hubei Agricultural Sciences

基　　金：广东省大学生创新创业项目(1134712075);广东省自然科学基金项目(S2011040001653)

摘　　要：通过生物信息学方法对水稻(Oryza sativa L.)蜡质合成相关基因OsCER4启动子序列和表达特性进行了分析;通过构建OsCER4启动子驱动的GUS融合表达载体,分析了OsCER4基因的时空表达;并分别以200 mmol/L Na Cl、100μmol/L ABA和1.0%H2O2处理水稻幼苗,通过半定量RT-PCR分析逆境胁迫下OsCER4的表达模式。启动子序列分析表明,OsCER4启动子中包括大量根、茎、叶肉等特异表达位点以及与干旱、冷、盐等多种逆境胁迫相关的调控序列。表达特征分析表明,OsCER4基因在愈伤组织、根、茎、叶中均有表达,在颖壳和子房中也有表达。Na Cl和PEG等逆境胁迫下,OsCER4基因表达量在不同处理时间有所增加,H2O2胁迫下,OsCER4基因表达量有所下降。In this study, the promoter and spatial expression of wax biosynthesis related gene OsCER4 in rice（Oryza sativa L.）was analyzed via bioinformatics,and the temporal and spatial expression of OsCER4 was analyzed by GUS histochemical staining. In addition, the expression of OsCER4 was analyzed by semi-quantitative RT-PCR when the seedlings were treated with stresses, including 200 mmol/L Na Cl,100 μmol/L ABA and 1.0% H2O2. The bioinformatics analysis showed that the promoter of OsCER4 gene（about 2 kb upstream of OsCER4） was predicted to contain important regulatory elements including drought, high salt, coldness,ABA,light regulation,copper,and oxygen responsive elements. The analysis of OsCER4 gene expression profiles revealed that OsCER4 was expressed in callus,roots,stems,leaves as well as glume and ovary. The analysis of OsCER4 gene in response to stresses showed that the expression of OsCER4 gene increased at different time when the seedlings were treated with Na Cl and PEG, however, the expression of OsCER4 gene in seedlings decreased when treated with H2O2.

关 键 词：水稻(Oryza SATIVA L.) OsCER4基因 启动子 表达 逆境 

分 类 号：Q78[生物学—分子生物学]