校园常见植物叶绿素提取方法比较及其含量测定  

Comparison on Different Extraction Techniques About Chlorophyll and Determination of Chlorophyll Content of Common Plants in Campus

作　　者：何启平[1] 陈莹[1] 

机构地区：[1]菏泽学院生命科学系,山东菏泽274015

出　　处：《黑龙江农业科学》2015年第10期117-120,共4页Heilongjiang Agricultural Sciences

基　　金：菏泽学院科研基金资助项目(XYJJKY-2)

摘　　要：为筛选提取植物叶绿素的有效方法,以校园中21种植物叶片为材料,应用80%丙酮研磨法、80%丙酮:95%乙醇(1∶1)混合液浸泡法及纯丙酮∶无水乙醇(1∶1)混合液浸泡法进行叶绿素提取,比较分析不同提取方法的差异及不同种类植物叶片叶绿素含量的差异。结果表明:混合液浸泡法效果较研磨法好,且纯丙酮:无水乙醇(1∶1)混合液浸泡法效果最好;并分析比较了21种校园常见植物叶绿素含量的差异与其生长习性的关系。In order to study the eflective method to extract chlorophyll in plants the chlorophyll of collected 21 kind of leaves in the campus were extracted with 80% acetone grinding method,80% acetone∶95% alcohol（1∶1）mixture soaking method and pure acetone∶anhydrous ethanol（1∶1）mixture soaking method,chlorophyll content were comparative analyzed by spectrophotometry.The results showed that the ordinary blade in a mixture method effect was good and pure acetone∶anhydrous ethanol（1∶1）mixture immerse worked the best with soaking for 24 hours.Comparison on the relationship between 21 kind of campus common plant chlorophyll content was analyzed with their growth habit,which were judged shade plants or sun plants.

关 键 词：叶绿素含量 丙酮研磨法 混合液浸泡法 阴生植物 阳生植物 

分 类 号：Q946[生物学—植物学]