溴氰菊酯对小鼠学习记忆及γ-氨基丁酸A受体α1和γ2亚单位表达的影响  

Effects of sub-chronic exposure to deltamethrin on the behavior of mice in learning and memory and expression of GABAA receptor α1 and γ2 subunits in the hippocampus of mice

作　　者：曹佩 李晨汐 冯永全 王小丹 马宁 徐海滨 

机构地区：[1]国家食品安全风险评估中心,北京100021

出　　处：《中华行为医学与脑科学杂志》2015年第10期885-887,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目（81202212）;农药风险评估综合配套技术研究项目（200903054-06）

摘　　要：目的研究溴氰菊酯（dehamethrin，DM）亚慢性染毒对小鼠学习记忆及海马组织γ-氨基丁酸A受体（GABAA）α1和γ2亚单位表达的影响。方法清洁级雌性昆明小鼠60只，分为溶剂对照组、低剂量染毒组、中剂量染毒组和高剂量染毒组，每组5只小鼠。后3组通过灌胃连续染毒60d。采用旷场试验评价小鼠的自发活动和探索行为；RQ—PCR技术检测海马组织中GABAA受体α1和γ2亚单位mRNA的表达。结果DM亚慢性染毒后，中剂量组中心区运动距离为（555．1±12．80）cm，高剂量组中心区运动距离为（516．4±11．88）cm，明显高于溶剂对照组（327．3±117．8）cm，差异具有统计学意义（P〈0．05）。高剂量组小鼠边缘区站立次数（27．9±9．9）次（F=4．117，P=0．010）和总的运动距离（3789．2±443．5）cm（F=2．914，P=0．042）也明显高于对照组小鼠边缘区站立次数（15．1±8．9）次和总运动距离（3211．3±379．8）cm，差异具有统计学意义。高剂量组和中剂量组小鼠海马组织中GABAA受体α1亚基因mRNA的相对表达水平显著低于溶剂对照组和低剂量组，差异具有统计学意义（F=8．508，P=0．001）；高剂量组小鼠海马组织中GABAA受体12亚基因mRNA的相对表达水平则明显低于其他三个组，差异具有统计学意义（F=6．738，P=0．002）。结论DM亚慢性染毒可影响小鼠的自发活动和探索行为，并抑制海马组织中GABAA受体d1和^y2亚基因mRNA的表达。Objective To study the effect of sub-chronic exposure to dehamethfin（DM） on the behavior of mice in learning and memory and expression of GABAA receptor α1 and γ2 subunits in the hippocampus of mice.Methods 60 female SPF Kunming mice were randomly divided into solvent control group,low-dose group, middle-dose group and high-dose group, 15 mice in each group.The latter three groups were exposured to deltamethrin for 60 days by gavage. Open field test （OFT） was applied to evaluate locomotor activity and exploratory behavior in mice. RQ-PCR was employed to measure the expression of GABAA receptorα1 and γ2 subunits in hip- pocampus of mice.Results After exposure to DM, the moving distance of the central area in the middle-dose group （ （555.1 ±12.8） cm） and high-dose group （ （ 516.4± 11.88 ） cm） was significantly higher than that in the solvent control group （ （327.3±117.8）cm, P〈0.05）. Numbers of standing in marginal area （F=4.117, P=0.010） and total movement distance （F= 2.914, P= 0.042） in the high-dose group （（27.9±9.9） times, （3211.3±379.8） cm） were also significantly higher than that in the solvent control group （ （ 15.1 ± 8.9） times, （ 3211.3± 379.8 ） cm）. The expression of GABAA receptor α1 subunit mRNA in the middle-dose group and high-dose group was significantly lower than that in the low-dose group and solvent control group （F= 8. 508, P= 0. 001 ） and the expression of GABAA receptor γ2 subunit mRNA in high-close group was significantly lower than that in the other groups （F= 6. 738, P= 0.002）. Conclusion Sub-chronic exposure to DM can damage the function of locomotor activity and exploratory behavior, and inhibit the expression of GABAA receptor α1 and γ2 subunits in the hippocampus of mice.

关 键 词：溴氰菊酯 亚慢性 学习记忆 Γ-氨基丁酸A受体 

分 类 号：R114[医药卫生—卫生毒理学]