微粒子化学发光法检测乙型肝炎病毒 e抗原的临床价值  

Clinical value of microparticle chemiluminescent detection of HBV e antigen

作　　者：刘涛 王兰兰 

机构地区：[1]河南省鹤壁市传染病医院检验科,458000

出　　处：《中国实用医刊》2015年第21期87-88,共2页Chinese Journal of Practical Medicine

摘　　要：目的：探讨乙型肝炎病毒e抗原（HBeAg）采用微粒子化学发光法（MCLIA）检测的临床价值。方法选取2013年4月至2014年4月收治的310例乙型肝炎患者，分别用MCLIA法与酶联免疫吸附法（ ELISA）对HBeAg进行检测。结果 HBeAg 的S／CO为＜1．00组、10．00～29．99组，≥30．00组三组标本时，应用MCLIA 法检验， MCLIA、ELISA法的符合率均＞95％，S／CO为这组标本为1．00～9．99时，MCLIA法和ELISA法，仅50％符合率。对大三阳抗病毒治疗患者行回顾分析，治疗中有HBeAg＋时，与治疗前相比，三者含量有可比性。结论 HBeAg采用MCLIA法检测，有较重要价值，与ELISA法比较，除灵敏度高外，在针对低含量HbeAg检测时，检出率也居较高水平，且可对HBeAg的变化动态、实时观察，使临床对抗病毒疗效可准确评估，值得临床广泛推广应用。Objective To investigate the clinical value of microparticle chemiluminescent（MCLIA） detection of HBV e antigen（HBeAg）.Methods From April 2013 to April 2014, 310 cases of hepatitis B were selected.The levels of HBeAg were detected by MCLIA method and enzyme-linked immunosorbent assay（ELISA）.Results HBeAg of S/CO was＆lt;1.00 group, 10.00-29.99 Group, when≥30.00 samples test application MCLIA, MCLIA, ELISA method in line with rates＆gt;95%, S/CO for this group of samples was 1.00 -9.99 when, MCLIA and ELISA, only 50 percent compliance rate.Antiviral therapy for patients with HBeAg retrospective analysis, the treatment when there HBeAg＋, compared with before treatment, they were comparable content.Conclusions HBeAg was detected by MCLIA method, and it is of impor-tant value, compared with the ELISA method, the sensitivity is high, aiming at low content of HbeAg detection, the detec-tion rate is among the highest in the higher level, and can be dynamically, changes on the HBeAg real time observation, the clinical efficacy against virus can be assessed accurately, is worthy of extensive promotion and application.

关 键 词：微粒子化学发光法 乙型肝炎病毒E抗原 临床价值 

分 类 号：R512.62[医药卫生—内科学]