辅助性T淋巴细胞17及相关细胞因子在急性乙型肝炎患者外周血中的动态变化及意义  

The dynamic changes and significance of T helper type 17 cells and cell-related cytokines in the peripheral blood of acute hepatitis B patients

作　　者：程雪[1] 单永业 罗亚文[1] 李佳[1] 

机构地区：[1]遵义医学院附属医院感染科,563003

出　　处：《中华传染病杂志》2015年第9期518-521,共4页Chinese Journal of Infectious Diseases

基　　金：贵州省科技厅社会发展攻关项目[黔科合SY字（2011）3048]

摘　　要：目的：了解急性乙型肝炎（AHB ）患者外周血 Th17细胞频率及相关细胞因子 IL‐21、IL‐22和IL‐23在病程各期的表达水平。方法流式细胞术检测10例AHB患者急性期、恢复早期和痊愈后外周血Th17细胞频率，ELISA检测IL‐21、IL‐22及IL‐23的水平。健康体检者10名作为对照。两组间比较采用 t检验，多组间比较采用单因素方差分析，相关性检验采用Pearson相关分析。结果健康对照组外周血 Th17细胞频率为（0．68±0．29）％，AHB患者急性期、恢复早期和痊愈后分别为（18．22±4．13）、（3．14±1．90）和（3．31±0．95）％，与健康对照组比较，差异有统计学意义（t值分别为13．405、4．047和8．342，均 P＜0．01）。健康对照组 IL‐21、IL‐22、IL‐23分别为（42．00±6．95）、（315．89±96．16）和（11．95±6．95） ng／L ，AHB患者急性期分别为（575．39±47．01）、（648．44±47．12）和（38．29±4．68） ng／L ，恢复早期分别为（366．50±33．74）、（405．04±47．12）和（25．10±4．69） ng／L ，痊愈后分别为（46．62±8．28）、（365．94±45．62）和（15．29±4．69） ng／L。AHB患者各期与健康对照组相比，IL‐21（t值分别为35．497、29．792和1．354，P值分别为＜0．01、＜0．01和0．193）、IL‐22（t值分别为9．820、2．632、1．487，P值分别为＜0．01、0．021和0．161）和IL‐23（t值分别为9．944、4．961和1．260， P值分别为＜0．01、＜0．01和0．226）差异均有统计学意义；3种细胞因子各期比较差异均有统计学意义（ F值分别为622．784、107．772和60．743，均 P＜0．01）。患者急性期 IL‐21、IL‐22、IL‐23水平与血清A L T水平呈正相关关系（ r值分别为0．655、0．666和0．673，均 P＜0．05）。相关性分析发现，Th17与IL‐21、IL‐22和IL‐23水平在急性期均呈正相关关系（ r值分别为0．879、0．866和0．879，均 P�Objective To investigate the expression of T helper type 17 cells （Th17） and cell‐related cytokines ,including interleukin （IL）‐21 ,IL‐22 ,IL‐23 in the peripheral blood of different clinical stages of patients with acute hepatitis B （AHB） .Methods Ten cases of AHB patients were enrolled .The frequency of Th17 cells in the three clinical stages （i .e .acute phase ,convalescent phase and resolved phase） were detected by flow cytometry . IL‐21 , IL‐22 and IL‐23 were measured by enzyme‐linked immunosorbent assay （ELISA） .Control group was composed of ten healthy subjects .The comparison between the two groups was done by t test and the differences among multiple groups were compared by one way ANOVA .Pearson correlation analysis was used for correlation analysis .Results The frequency of Th17 in healthy controls was （0 .68 ± 0 .29）% ,while those in acute phase ,convalescent phase and resolved phase of AHB patients were （18 .22 ± 4 .13）% , （3 .14 ± 1 .90 ）% and （3 .31 ± 0 .95 ）% , The differences between the two groups were significant （t= 13 .405 ,4 .047 and 8 .342 , respectively ;all P〈 0 .01） .The levels of IL‐21 ,IL‐22 and IL‐23 in healthy controls were （42 .00 ± 6 .95） ,（315 .89 ± 96 .16） and （11 .95 ± 6 .95） ng/L ,respectively .Those in acute phase of AHB patients were （575 .39 ± 47 .01） ,（648 .44 ± 47 .12） and （38 .29 ± 4 .68） ng/L ,respectively ,those in convalescent phase were （366 .50 ± 33 .74） ,（405 .04 ± 47 .12） and （25 .10 ± 4 .69） ng/L ,respectively ,while those in resolved phase of AHB patients were （46 .62 ± 8 .28） ,（365 .94 ± 45 .62） and （15 .29 ± 4 .69） ng/L , respectively .Compared with healthy controls ,t values of the levels of IL‐21 in three different phases of AHB patients were 35 .497 ,29 .792 and 1 .354 with P value of 〈0 .01 ,〈0 .01 and 0 .193 ,respectively ;those of IL‐22 were 9 .820 ,2 .632 and 1 .487 with P value of 〈 0 .01 ,0 .021 and 0 .161 ,respective

关 键 词：肝炎 乙型 急性 辅助性T淋巴细胞17 白细胞介素-21 白细胞介素-22 白细胞介素-23 

分 类 号：R512.62[医药卫生—内科学]