普通小麦和短柄草AGAMOUS LIKE 6基因RNA干扰载体的构建  

RNA Interference Vector Construction of AGL6 Genes in Bread Wheat and Brachypodium distachyon

作　　者：李海峰[1,2,3] 窦艳华[1] 刘楠[1] 韩德俊[1] 

机构地区：[1]西北农林科技大学旱区作物逆境生物学国家重点实验室,陕西杨凌712100 [2]西北农林科技大学农学院,陕西杨凌712100 [3]新疆农业职业技术学院,新疆昌吉831100

出　　处：《西北农业学报》2015年第10期22-27,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：西北农林科技大学引进人才科研启动费(Z111021201);陕西省自然科学基金项目(2014JM3065);中央高校基本科研业务费(2014ZZ009)~~

摘　　要：AGL6(AGAMOUS LIKE6)基因亚家族是植物MADS-BOX基因家族一个古老的亚家族,近年的研究表明,禾本科植物玉米(Zea maize)AGL6基因ZAG3、水稻(Oryza sativa)AGL6基因OsMADS6参与调控花的发育。OsMADS6还影响种子的发育。普通小麦(Triticum aestivum)和短柄草(Brachypodium distachyon)中AGL6基因的功能还不清楚。为解析小麦和短柄草AGL6基因的功能奠定基础,运用RT-PCR等分子生物学方法,克隆TaAGL6基因的cDNA序列,构建RNA干扰载体,同时分析TaAGL6基因的表达模式。结果表明:成功构建TaAGL6和BdAGL6 RNA干扰载体,小麦中有3个同祖AGL6基因,分别位于A、B、D基因组上,它们除了在花序中高水平表达外,在幼苗的根中也有一定表达。AGL6（AGAMOUS LIKE 6）is an ancient sub-family of MADS-BOX gene family in plants.Recent results in maize and rice showed AGL6 genes participate in regulating flower development.Rice AGL6 gene OsMADS6 affects seed development too.Functions of AGL6 genes in bread wheat（Triticum aestivum）and new model plant Brachypodium distachyon remain unclear.For laying a foundation to elucidate the biological function of TaAGL6 and BdAGL6,we cloned the cDNA sequence of TaAGL6 and constructed RNA interference（RNAi）vectors of TaAGL6 by employing RT-PCR and other molecular biological technologies in this research.Meanwhile,the expression pattern of TaAGL6 was analyzed.In addition to successful construction of TaAGL6 and BdAGL6 RNA interference vectors,results showed that there are three homoeologous AGL6 genes in A,B and C genome respectively in bread wheat.They are expressed in flowers strongly,and also expressed in roots of seedlings.

关 键 词：小麦 短柄草 AGL6基因 RNA干扰 

分 类 号：Q943.2[生物学—植物学]