外源亚精胺对盐胁迫下菠菜叶绿素合成前体含量的影响  

Effects of Exogenous Spermidine on Chlorophyll Precursors Content of Spinach Plants under Salt Stress

作　　者：王颖[1,2] 郭世荣[1,2] 束胜[1,2] 刘芳[1,2] 刘涛[1,2] 孙锦[1,2] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]南京农业大学(宿迁)设施园艺研究院,江苏宿迁223800

出　　处：《西北植物学报》2015年第10期2026-2034,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：现代农业产业技术体系建设专项资金(CARS-25-C-03);江苏省农业"三新"工程项目(SXGC[2015]321;SXGC[2015]270);南京农业大学植物生产国家级实验教学中心开放性实验项目(ZKF201302);江苏省重大创新载体建设项目(BY2011012);江苏省前瞻性联合研究项目(BY2013071)

摘　　要：采用营养液水培的方法,以耐盐性较弱的菠菜(Spinacia oleracea L.)品种‘全能菠菜’为试材,研究外源亚精胺(Spd)对盐胁迫下菠菜生长及叶绿素前体含量的影响。结果显示:(1)菠菜植株在250mmol·L-1 NaCl胁迫下生长受到显著抑制,单株总鲜重、总干重、地上部鲜重、地上部干重、地下部鲜重和地下部干重分别较对照降低57.23%、53.08%、63.14%、55.05%、42.22%和42.86%,叶面喷施1.0mmol·L-1Spd使其分别比盐胁迫处理提高62.83%、71.19%、60.57%、71.74%、70.31%和69.23%;(2)250 mmol·L-1盐胁迫使菠菜叶片叶绿素a(Chl a)、叶绿素b(Chl b)及总叶绿素Chl(a+b)含量分别较对照显著降低42.31%、54.55%和44.53%,叶面喷施1.0mmol·L-1 Spd使其分别较单纯盐胁迫处理显著提高46.24%、51.85%和47.11%;(3)盐胁迫使菠菜叶片Chl a、Chl b、叶绿素酸(Pchl)、镁原卟啉Ⅸ(Mg-protoⅨ)、原卟啉Ⅸ(ProtoⅨ)和尿卟啉Ⅲ(UroⅢ)含量均显著降低,而使胆色素原(PBG)和δ-氨基酮戊酸(ALA)含量升高,而叶片Chl a、Chl b、Pchl、Mg-protoⅨ、ProtoⅨ和UroⅢ含量在施用外源Spd后均显著提高,而其PBG和ALA含量有所降低。可见,盐胁迫条件下,菠菜叶片叶绿素合成受阻,受阻位点在PBG向UroⅢ的转化过程,外源Spd能够缓解盐胁迫下菠菜叶片叶绿素合成受阻的程度,促进叶绿素合成,从而提高叶绿素含量。Effect of exogenous spermidine（Spd）on growth and chlorophyll precursors content of spinach plants under NaCl stress was studied by hydroponics using‘cv.Quanneng＇of spinach,a sensitive cultivar to salt.The results showed that：（1）growth of spinach plants was inhibited significantly by 250mmol·L-1NaCl,as a result of total fresh weight,total dry weigh,shoot fresh weight,shoot dry weigh,root fresh weight and root dry weigh of spinach plants were significantly decreased by 57.23%,53.08%,63.14%,55.05%,42.22% and 42.86%,respectively,as compared with control.However,plant growth was enhanced by 1.0mmol·L-1 Spd,those indexes increased by 62.83%,71.19%,60.57%,71.74%,70.31%and 69.23%,respectively,as compared with salt stress treatment.（2）Chlorophyll a（Chl a）,chlorophyll b（Chl b）and total chlorophyll[Chl（a＋b）]contents in leaves of spinach plants were decreased by 250mmol·L-1 salt stress to 42.31%,54.55% and 44.53%,respectively,but those were increased by 1.0mmol·L-1 Spd to 46.24%,51.85% and 47.11%,as compared with salt stress treatment.（3）Chlorophyll precursors such as Chl a,Chl b,protochlorophyll（Pchl）,Mg-protoporphyrin Ⅸ（Mg-protoⅨ）,protoporphyrin Ⅸ（ProtoⅨ）and uroorphyrinogenⅢ（UroⅢ）were decreased by salt stress,but were increased by Spd.However,others such asδ-aminolevulinic acid（ALA）and porphobilinogen（PBG）were enhanced by salt stress,but were suppressed by Spd.These results suggested that chlorophyll synthesis process from PBG to UroⅢ was inhibited by salt stress,and this inhibition was alleviated by exogenous Spd,as a result of chlorophyll increasing.

关 键 词：外源亚精胺 盐胁迫 菠菜 叶绿素前体 

分 类 号：Q945.78[生物学—植物学]