乙酰半胱氨酸活性炭微囊治疗大鼠肝纤维化的实验研究  

Role of treatment on liver fibrosis of activated carbon N-acetylcysteine microcapsule

作　　者：方红英[1] 庄让笑[1] 席建军[1] 孙晶晶[1] 邵益丹[1] 史婷婷[1] 潘旭旺[1] 王福根[1] 

机构地区：[1]浙江中医药大学附属杭州西溪医院

出　　处：《中国临床药理学与治疗学》2015年第9期976-980,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：杭州市科技计划项目(20110833B17)

摘　　要：目的:研究乙酰半胱氨酸活性炭微囊(activated carbon N-acetylcysteine microcapsule,ACNAC)治疗大鼠肝纤维化的疗效。方法:以二甲基亚硝胺(dimethylnitrosoamine,DMNA)制备大鼠肝纤维化模型。将大鼠分为正常组、模型组、ACNAC高、中、低剂量组、水飞蓟宾(silibinin,SM)组和乙酰半胱氨酸(N-acetylcysteine,NAC)组。ACNAC以20、40、80 mg/kg(按乙酰半胱氨酸计算)剂量每日分别灌胃进行肝纤维化治疗,给药8周后处死大鼠,通过血液生化、肝组织各项指标的测定和病理切片观察肝纤维化情况,对ACNAC治疗肝纤维化作用进行评价。结果:DMNA造模结束后,大鼠肝组织病理结果显示其肝纤维化处于Ⅲ-Ⅳ期;与模型组比较,ACNAC治疗组血清学指标显著改善;肝组织羟脯氨酸(hydroxyproline,Hyp)、丙二醛(methane dicarboxylic aldehyde,MDA)明显下降,超氧化物歧化酶(superoxide dismutase,SOD)活力显著增加;ACNAC各组Massion染色结果显示肝纤维化好转,尤其是高剂量组肝组织改善明显。结论:ACNAC对大鼠肝纤维化具有较好的治疗作用,疗效优于乙酰半胱氨酸(NAC)对照组。AIM： To research the curative effect on treatment of liver fibrosis of activated carbon Nacetylcysteine microcapsule（ ACNAC）. METHODS： Prepare liver fibrosis models by dimethylnitrosoamine（ DMNA） gavage on male SD rats. Rats were assigned into control group, model group,high-dose,mediun-dose,low-dose group,silibinin（ SM） group and N-acetylcysteine（ NAC） group.Treated on liver fibrosis of the group as a unit to gavage different daily dose of ACNAC（ 20,40,80 mg / kg） according to be calculated by NAC. All the rats were killed after 8 weeks of administration,evaluated the role in treatment of ACNAC of liver fibrosis by blood biochemistry,hepatic tissue indicators and pathological section. RESULTS： The result showed that the hepatic fibrosis was in III-IV stage.Compared with the model group,ACNAC could significantly improve the levels in serum. It also could decrease Hyp,MDA and increase the level of SOD.The result showed that the every massion dye group of ACNAC made liver fibrosis better,especially high dose group. CONCLUSION： ACNAC have a better therapeutic effect on rat liver fibrosis induced the role of treatment is better than NAC control group by DMNA.

关 键 词：乙酰半胱氨酸活性炭微囊 乙酰半胱氨酸 肝纤维化 

分 类 号：R965.2[医药卫生—药理学]