细胞浓度比对剪切流中肿瘤细胞黏附的影响  

The effect of concentration ratio on tumor cell adhesion in shear flow

作　　者：傅怡[1] 王洋[2] 白帆[1] 吴洁[1] 孙仁[1] 丁祖荣[1] 董澄[1,3] 

机构地区：[1]上海交通大学船舶海洋与建筑工程学院,水动力学教育部重点实验室,上海200240 [2]复旦大学附属上海市第五人民医院,泌尿外科,上海200240 [3]Department of Biomedical Engineering, the Pennsylvania State University, PA 16802, USA

出　　处：《医用生物力学》2015年第5期392-396,共5页Journal of Medical Biomechanics

基　　金：国家自然科学基金项目(11302129)

摘　　要：目的研究不同白细胞与肿瘤细胞浓度比对剪切流环境中肿瘤细胞在内皮细胞上黏附过程的影响。方法在平行平板流动腔中加入不同浓度比的白细胞和肿瘤细胞,分析不同剪切率下(50、100、200 s-1)肿瘤细胞在内皮细胞上瞬时黏附和累积黏附的变化。结果细胞浓度比为3∶1时,肿瘤细胞在内皮细胞上的瞬时黏附和累积黏附均比细胞浓度比为1∶1时有显著提升,且在高剪切率(100、200 s-1)下更为明显。在观察的5 min内,随着剪切率的增加,细胞浓度比增大对肿瘤细胞黏附产生影响的时间点有所提前。结论在剪切流中白细胞浓度增加对肿瘤细胞在内皮细胞上的黏附起到促进作用,结果为研究肿瘤在血管中转移和靶向治疗提供有意义的探索。Objective To study the different effects from different concentration ratios of polymorphonuclear neu- trophil （PMN） to tumor cell （TC） on the process of tumor cell adhesion to endothelial cell （EC） in shear flow. Methods PMNs and TCs with different concentration ratios （PMN-TC ratio） were added into the parallel plate flow chamber, and changes in the numbers of transient and accumulative adhered TCs on ECs at different shear rates （50 s^-1 ,100 s^-1 ,200 s^-1 ） were analyzed. Results The transient and accumulative adhesion of TCs on ECs at PMN-TC ratio of 3 ： 1 significantly increased as compared to that at PMN-TC ratio of 1 ： 1, especially under high shear flow condition （ 100 s^-1 and 200 s^-1 ）. Moreover, in the 5 minute-observation period, the effect of PMN-TC ratio on TC adhered to ECs occurred earlier when the shear rate increased. Conclusions The increase of PMN-TC concentration ratio can promote TC adhesion to ECs in shear flow, and the research findings provide significant references for studying TC metastasis in blood vessels and the target therapy of tumors.

关 键 词：浓度比 内皮细胞 白细胞 肿瘤细胞 剪切率 细胞黏附 

分 类 号：R318.01[医药卫生—生物医学工程]