燕麦D基因组特异标记开发  

Development of D Ggenome-specific Marker in Oat

作　　者：翟志文[1] 柴国师[2] 候莎莎[1] 剌士潇 靳艳婷 贾举庆[1] 

机构地区：[1]山西农业大学农学院,山西太谷030801 [2]电子科技大学生命科学技术学院,成都610054

出　　处：《农学学报》2015年第10期88-92,共5页Journal of Agriculture

基　　金：山西省普通高等学校大学生创新创业训练项目"野生燕麦基因组特异标记的开发与应用"(2012075);山西农业大学大学生科技创新项目"野生燕麦基因组特异标记的开发与应用"(10-004);山西农业大学引进人才科研启动项目"燕麦基因组中反转录转座子的分离与应用"(XB2010009)

摘　　要：为了提高燕麦分子育种的效率,旨在开发燕麦D基因组特异分子标记。先利用300条10碱基随机引物对9种燕麦种材料进行随机多态性扩增(random amplified polymorphic DNA,RAPD),扩增出燕麦D基因组特异性条带,命名为OPAB011287,经Blast比对后发现该条带为一反转座子重复序列,根据此条带序列设计特异性引物,建立更简便稳定的SCAR标记。结果发现该SCAR标记可以准确特异的检测到9种试验材料中含有D基因组的燕麦品种,结论表明该标记可用于燕麦分子标记辅助育种,有效服务于燕麦优良品种培育。The research aims to improve the efficiency of molecular breeding and develop D- Genome-specific markers in oat.Firstly,random amplified polymorphic DNA（RAPD） analysis was performed on 9 oat varieties with 300 random 10-based primers.A repetitive sequence of 1287 bp,named OPAB01_（1287） was identified in the D genome of oat.BLAST search revealed that the sequence of OPAB01_（1287） was highly homologous to a part of a retrotransposon.Based on the sequence of OPAB01_（1287）,a pair of sequence-characterized amplified region primers（SCAR） was designed which was more convenient and stable.And the results showed that SCAR marker was specific to target oat with D genome,which provided a powerful tool for oat marker assisted breeding and the better service for the breeding of oat.

关 键 词：燕麦 D基因组 重复序列 分子标记 

分 类 号：S512.6[农业科学—作物学]