植物Dicer-like功能的研究进展  

Research Progress of Dicer-like in Plants

作　　者：李蕊[1] 王恬[1] 文莉薇 朱鸿亮[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《中国农学通报》2015年第30期210-214,共5页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金面上项目"番茄果实成熟相关Dicer-like 2c的调控机制研究"(31471921);中央高校基本科研业务费专项资金"番茄Argonaute基因家族调控果实发育成熟的机制研究"(2014RC006)

摘　　要：Dicer是一种核酸内切酶,是RNA酶Ⅲ(RNaseⅢ)家族成员之一。植物中Dicer的同源蛋白称为Dicer-like(DCL)。通常情况下,Dicer蛋白由DEAD box、RNA解旋酶结构域、DUF283、PAZ、RNaseⅢ和双链RNA结合结构域(ds RNA binding domain,ds RBD)6部分组成,各个结构域之间紧密联系,共同作用。为了进一步深化对植物中DCL功能的认识,本研究归纳了DCL的蛋白结构域、DCL在小RNA生成中的剪切作用以及几种模式植物中DCL的生化作用,提出了不同类型的DCL在小RNA合成机制中相对独立又密切联系的作用。DCL识别何种RNA底物以及对底物剪切位置的确定,将会成为研究Dicer生化功能的重点内容。Dicer was an endonuclease, which was a member of the RNaseⅢ family. Its homologous protein wascalled Dicer-like（DCL） in plants. Generally, Dicer was composed of six domains, DEAD box, helicase-C,DUF283, PAZ, RNase III, and ds RBD. The six domains contacted closely to each other and worked together.In order to improve the understanding of the functions of DCL in plants, this article summarized the proteindomains of DCL, their crucial roles in small RNA synthesis and the biochemical function in several modelplants, demonstrated that different types of DCL proteins showed relatively independent and closely linkedrelationships in small RNA production. DCL could discern the kind of RNA substrates and the exact cut sites,and would be the focus of biochemical function study of Dicers.

关 键 词：DICER Dicer-like 结构域 小RNA 剪切 

分 类 号：Q71[生物学—分子生物学]