普伐他汀对激素性兔股骨头坏死BMP2 mRNA及Cbfα1 mRNA水平的影响  

Influence of pravastatin on the level of BMP2 mRNA and Cbfα1 mRNA of rabbit femoral head necrosis induced by hormone

作　　者：乐锦波[1,2] 梁杰[1,2] 杜远立[1,2] 向选平[1,2] 

机构地区：[1]三峡大学人民医院 [2]宜昌市第一人民医院骨科,湖北宜昌443000

出　　处：《现代医学》2015年第9期1124-1127,共4页Modern Medical Journal

摘　　要：目的:观察普伐他汀对激素性兔股骨头坏死骨形态发生蛋白2(BMP2)mRNA及核心结合因子(Cbfα1)mRNA水平的影响。方法:将45只实验白兔根据数字表法随机分为对照组(n=15)、模型组(n=15)和普伐他汀组(n=15)。模型组和普伐他汀组均构建激素性兔股骨头坏死模型;普伐他汀组予以普伐他汀灌胃,对照组和模型组仅予以生理盐水灌胃。比较各组兔股骨头组织切片骨小梁、骨陷窝及血管数等指标,采用反转录-聚合酶链反应(RT-PCR)法检测造模后第8、12、16周时BMP2 mRNA及Cbfα1mRNA表达水平。结果:普伐他汀组血管数量及其直径均明显大于对照组和模型组(P<0.05),而骨陷窝空缺百分率低于其他两组(P<0.05);普伐他汀组造模后第8、12、16周时BMP2 mRNA及Cbfα1 mRNA表达水平均明显高于对照组和模型组(P<0.05),且随治疗时间延长,BMP2 mRNA及Cbfα1 mRNA表达水平明显升高(P<0.05);BMP2 mRNA表达水平与Cbfα1 mRNA表达水平之间呈正相关(P<0.05)。结论:普伐他汀可明显改善激素性兔股骨头坏死组织病理学表现,可能通过调节BMP2 mRNA、Cbfα1 mRNA而发挥其作用。Objective： To observe the effect pravastatin on the level of bone morphogenetic protein 2（ BMP2）mRNA and Cbfα1 mRNA of rabbit femoral head necrosis induced by hormone. Methods： Forty-five experimental rabbits were randomly divided into control group（ n = 15）,model group（ n = 15） and pravastatin group（ n = 15）according to the digital table method. The rabbits of model group and pravastatin group were build femoral head necrosis induced by hormone model,and the rabbits in pravastatin group were lavage by pravastatin,in control group and model group were only lavage by saline. The bone trabecular,bone pit and the number of blood vessels of rabbit femoral head biopsy between the groups were compared,and the expression level of BMP2 mRNA and Cbfα1 mRNA 8,12,16 weeks after model build were detected by RT-PCR method. Results： The number and diameter of blood vessels in pravastatin group were significantly greater than those of the control group and model group（ P 〈 0. 05）,while the percentage of bone pit openings was lower than those of other two groups（ P 〈 0. 05）.The expression level of BMP2 mRNA and Cbfα1 mRNA 8,12,16 weeks after model build in pravastatin group were significantly higher than those of control group and model group（ P 〈 0. 05）,and with the extension of treatment time,the expression level of BMP2 mRNA and Cbfα1 mRNA increased significantly（ P 〈 0. 05）. The expression level of BMP2 mRNA had a positive correlation with the expression level of Cbfα1 mRNA（ P 〈 0. 05）.Conclusion： Pravastatin can obviously improve the tissue pathology of sexual hormone rabbit femoral head necrosis,and it may be exert its pharmacological effects by adjusting the BMP2 mRNA,Cbfα1 mRNA.

关 键 词：普伐他汀 股骨头坏死 骨形态发生蛋白2 核心结合因子 

分 类 号：R681[医药卫生—骨科学] R-33[医药卫生—外科学]