舟山眼镜蛇毒α-神经毒素致突变性研究(英文)  

Studies on mutagenicity of α- neurotoxin from Naja naja atra venom

作　　者：韦传宝[1,2] 李更青 梅元元[1] 高总结 陶用鑫 费鑫[1] 

机构地区：[1]皖西学院生物与制药工程学院,安徽六安237012 [2]皖西学院抗体制备及其质量检测中心,安徽六安237012

出　　处：《中国新药与临床杂志》2015年第10期764-768,共5页Chinese Journal of New Drugs and Clinical Remedies

基　　金：supported by Natural Scientific Foundation of Anhui Province(1408085MC43);Natural Scientific Foundation of Anhui Province Education Commission(KJ2013A264)

摘　　要：目的对舟山眼镜蛇毒α-神经毒素的致突变性进行研究,为临床应用的安全性提供依据。方法应用中国仓鼠肺成纤维细胞CHL染色体畸变试验和Ames试验检测舟山眼镜蛇毒α-神经毒素是否有致突变性。结果 Ames试验:对于突变株TA97、TA100和TA102,α-神经毒素浓度达到或者超过2.56μg·m L-1,Ames试验阳性(P<0.05);对于突变株TA98,α-神经毒素浓度达到或者超过5.12μg·m L-1,Ames试验阳性(P<0.05)。但回复突变和畸变率与神经毒素的浓度不呈线性关系,也与是否有活化剂S9无关联。染色体畸变试验:α-神经毒素浓度达到或者超过0.64μg·m L-1,CHL细胞染色体畸变为阳性(P<0.05),但畸变率与神经毒素的浓度不呈线性关系,也与是否有活化剂S9无关联。结论舟山眼镜蛇毒α-神经毒素临床剂量为1μg·kg·d-1时在致突变性上是安全的。AIM To estimate the mutagenicity of α- neurotoxin purified from Naja naja atra venom for clinical safe usage. METHODS The potential mutagenicity of α- neurotoxin from Naja naja atra venom was studied through Ames test using histidine requiring Salmonella typhimurium indicator strains TA97, TA98, TA100 and TA102and Chinese hamster lung fibroblast chromosome aberration test. RESULTS Ames test was positive（P〈0.05） when the concentration of α- neurotoxin ≥ 2.56 μg·m L-1for TA97, TA100 and TA102, and ≥ 5.12 μg·m L-1for TA98. There was no linear relationship between the concentration and back mutation rate and no difference between groups with S9（ metabolic activation system mixture） and groups without S9. Chromosomal aberration test was positive at 0.64 μg· m L-1（ P〈0.05）. There is no linear relationship between the concentration and chromosomal aberration rate and no difference between groups with S9 and groups without S9.CONSLUSION The α- neurotoxin from Naja naja atra venom is safe on the mutagenicity as a drug at clinical dosage of 1 μg·kg·d-1.

关 键 词：舟山眼镜蛇 眼镜蛇神经毒素类 AMES试验 染色体畸变 诱变力试验 

分 类 号：R996.3[医药卫生—毒理学]