CYP1A1基因多态性与急性淋巴细胞白血病儿童大剂量甲氨蝶呤毒副反应的关系  

Association of CYP1A1 gene polymorphism with adverse reactions of high-does methotrexate in children with acute lymphocytic leukemia

作　　者：邹泽巧 岳丽杰[2] 任艳飞[2] 

机构地区：[1]遵义医学院珠海校区研究生院,广东省珠海市519090 [2]深圳市儿童医院儿科研究所,广东省深圳市518026

出　　处：《实用医学杂志》2015年第18期2960-2962,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金(编号:30471830);深圳市科技计划重点项目(编号:201101011)

摘　　要：目的:探讨细胞色素氧化酶P4501A1(CYP1A1)基因多态性与急性淋巴细胞白血病(ALL)儿童大剂量甲氨蝶呤(HD-MTX)化疗后毒副作用的关系。方法:用RT-PCR-变性梯度凝胶电泳(DGGE)及DNA测序技术对51例ALL儿童CYP1A1进行多态性筛查,统计患儿HD-MTX毒副作用表现,分析两者相关性。结果:仅筛查到一个多态位点,即A4889G,4889A/G各基因型与患儿毒副作用发生均无关(P>0.05),高危型患儿较标危型患儿更易发生血小板减少(P<0.05)。结论:CYP1A1 A4889G多态性与ALL儿童HD-MTX化疗后各毒副反应无关,血小板减少的发生可能与患儿危险度相关。Objective To investigate the association between CYP1A1 gene polymorphism and toxicities related to high-does methotrexate of childhood acute leukemia. Methods The SNPs were detected by reverse transcriptional（RT）-PCR-denaturing gradient gel elelctrphoresis combined with direct sequencing in 51 children with acute leukemia. Toxicities were collected thereby. Results Only one SNP,A4889 G, was screened in CYP1A1. A4889 G polymorphism was not associated with all the toxicities（P〉0.05）. High-risk ALL children were more likely to increase the risk of thrombocytopenia compared with standard-risk ALL（P〈0.05）.Conclusions CYP1A1 A4889 G polymorphism may be not association with all toxicities after HD-MTX,but the thrombocytopenia may be relevant to the risk degree of ALL children.

关 键 词：细胞色素氧化酶P4501A1 多态性 甲氨蝶呤 急性淋巴细胞白血病 

分 类 号：R733.71[医药卫生—肿瘤]