T140体内靶向阻断SDF-1/CXCR4信号通路对SDF-1及MMPs的影响  

The study of targeted blocking SDF-1 / CXCR4 signaling pathway in vivo with T140 on SDF-1 and MMPs levels

作　　者：王坤[1] 赵沣凯 李彦林[1] 余洋[1] 高寰宇 肖渝[1] 李龙滕 闫祥甲 贾迪[1] 

机构地区：[1]昆明医科大学第一附属医院运动医学科,650031 [2]山东省枣庄市枣矿集团公司中心医院,277800

出　　处：《实用医学杂志》2015年第19期3133-3136,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81460340);云南省创新团队项目(编号:2014HC018);云南省医学学科带头人培养项目(编号:D-201207)

摘　　要：目的:明确T140体内靶向阻断SDF-1/CXCR4信号通路对基质细胞衍生因子-1(SDF-1)及基质金属蛋白酶(MMPs)的影响,探讨T140延缓软骨退变的作用。方法:健康9月龄雄性Hartley豚鼠36只,随机分成A组(实验组)、B组(实验对照组)、C组(空白对照组),每组12只。2、4、6、8、10、12周时,ELISA法测血清中SDF-1含量。12周时,RT-PCR法测关节软骨内基质金属蛋白酶(MMP-3、MMP-9、MMP-13)m RNA的表达。结果:A组血清中SDF-1含量逐渐降低,B、C组逐渐升高,同一时间比较,A组与B、C组有明显差异(P<0.05);A组MMP-3、9、13 m RNA的表达量低于B、C组,差异有统计学意义(P<0.05)。结论:T140靶向阻断SDF-1/CXCR4信号通路,减少血清中SDF-1的分泌并降低软骨内MMPs m RNA的表达量,从而减缓关节软骨的退变。Objective To explore the effect of T140 on SDF-1同 and MMPs levels through targeted blocking SDF-I/CXCR4 signaling pathway, and to investigate the function of T140 to prevent from cartilage degeneration. Methods Thirty-six 9-month-old male healthy Hartley guinea pigs were divided into three groups： experimental group（group A,n = 12）,experimental control group（group B,n = 12） and blank control group （group C, n = 12）. In the 2nd, 4th, 6th, 8th, 10th, 12th week, the levels of SDF-1 in serum were quantified with ELISA. In the 12th week, mRNA levels of MMP-3,MMP-9 and MMP-13 in articular cartilages were measured with RT-PCR. Results The serum levels of SDF-1 of the group A decreased gradually but increased in group B and C. Group A had a statistical significance compared with group B and C at the same time point（P 〈 0.05）.The mRNA levels of MMPs in group A were lower than group B and C（P 〈 0.05）. Conclusion T140 could block the SDF-1/CRCR4 signaling pathway and decrease the secretion of SDF-1 and mRNA expression levels of MMPs and reduce the cartilage degeneration.

关 键 词：T140 SDF-1/CRCR4信号通路 基质金属蛋白酶类 软骨退变 

分 类 号：R684.3[医药卫生—骨科学]