GC法测定鱼腥草挥发油中α-蒎烯、β-蒎烯等7种成分的含量  

GC simultaneous determination of the contents of seven constituents in volatile oil from Houttuyniae Herba

作　　者：刘玉红[1] 李青苗[1] 黄志芳[1] 刘云华[1] 易进海[1] 

机构地区：[1]四川省中医药科学院,成都610041

出　　处：《药物分析杂志》2015年第10期1810-1814,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：四川省科技计划课题(2014SZ0071)

摘　　要：目的：用GC同时测定鱼腥草挥发油中α-蒎烯、β-蒎烯、β-月桂烯、柠檬烯、4-萜品醇、乙酸龙脑酯和甲基正壬酮7种成分的含量。方法：色谱柱：HP-5（30 m×0.32 mm×0.25μm）石英毛细管柱;进样口温度：240℃;检测器：FID;检测器温度：260℃;柱温：70℃为起始温度,保持5 min,以4℃·min-1升温至140℃,保持5 min,再以20℃·min-1升温至250℃;载气：氮气;流速：1.0 m L·min-1;进样方式：分流,分流比20∶1;萘作为内标。结果：α-蒎烯、β-蒎烯、β-月桂烯、柠檬烯、4-萜品醇、乙酸龙脑酯和甲基正壬酮色谱峰分离良好;进样浓度分别在0.009 8～2.5、0.012～3.1、0.008 0～2.0、0.012～2.9、0.009 1～2.3、0.009 3～2.3、0.009 9～2.5 mg·m L-1范围内线性关系良好;平均回收率（n=6）分别为99.2%、99.7%、98.2%、100.3%、98.6%、101.4%、99.4%,RSD分别为1.03%、0.95%、1.31%、1.48%、2.03%、1.87%、1.24%。对5批挥发油进行了含量测定,上述7个组分的含量分别为5.22%～11.9%、11.3%～29.4%、5.56%～13.8%、3.38%～4.13%、0.71%～6.27%、2.43%～4.02%、17.0%～27.5%。结论：建立的鱼腥草挥发油GC含量测定方法简便、快速、准确,为鱼腥草挥发油质量综合评价提供了科学依据。Objective： To establish a method for simultaneous determination of seven constituents（ α-pinene,β-pinene,β-myrcene,limonene,4-terpineol,bornyl acetate,and methyl n-nonylketone） in volatile oil from Houttuyniae Herba by GC. Methods： The sample was split in the 240 ℃ injection port with 20∶ 1 split ratio,and separated on a DB-5（ 30 m × 0. 32 mm × 0. 25 μm film thickness） fused silica column with nitrogen as the carrier gas,the gas speed was 1. 0 m L·min- 1. The temperature program was as follows： 70 ℃ for 5min,then 4 ℃·min- 1to 140 ℃,keep 5 min,20 ℃·min- 1to 250 ℃. The FID was used as detector and the FID temperature was set at 260 ℃.Naphthalene was used as internal standard. Results： The chromatographic peaks of α-pinene,β-pinene,β-myrcene,limonene,4-terpineol,bornyl acetate,and methyl n-nonylketone had good separation. Good linearity of the seven components were obtained within the range of 0. 009 8- 2. 5 mg·m L- 1（ r = 0. 999 9）,0. 012- 3. 1 mg·m L- 1（ r =0. 999 9）,0. 008 0-2. 0 mg·m L- 1（ r = 0. 999 9）,0. 012-2. 9 mg·m L- 1（ r = 0. 999 9）,0. 009 1-2. 3 mg·m L- 1（ r = 0. 999 9）,0. 009 3-2. 3 mg·m L- 1（ r = 0. 999 9） and 0. 009 9-2. 5 mg·m L- 1（ r = 0. 999 9）,respectively; the average recoveries of α-pinene,β-pinene,β-myrcene,limonene,4-terpineol,bornyl acetate,and methyl n-nonylketone were 99. 2%,99. 7%,98. 2%,100. 3%,98. 6%,101. 4%,and 99. 4%; and the RSDs were 1. 03%,0. 95%,1. 31%,1. 48%,2. 03%,1. 87%,and 1. 24%,respectively. The contents of α-pinene,β-pinene,β-myrcene,limonene,4- terpineol,bornyl acetate,and methyl n- nonylketone in five batches of samples were 5. 22%-11. 9%,11. 3%- 29. 4%,5. 56%- 13. 8%,3. 38%- 4. 13%,0. 71%- 6. 27%,2. 43%- 4. 02% and 17. 0%- 27. 5%respectively. Conclusion： The method is simple,rapid,accurate,and provides a scientific basis for quality assessment of volatile oil from Houttuyniae Herba.

关 键 词：鱼腥草 Α-蒎烯 Β-蒎烯 β-月桂烯 柠檬烯 4-萜品醇 乙酸龙脑酯 甲基正壬酮 挥发油含量测定 气相色谱 

分 类 号：R917[医药卫生—药物分析学]