外周血CD14^+CD16^+单核细胞在维持性血液透析患者中的表达  

Expression of CD14^+CD16^+ monocytes in peripheral blood of patients undergoing maintenance hemodialysis

作　　者：缪立英[1] 何小舟[1] 刘金凤[1] 金丽娜[1] 

机构地区：[1]苏州大学附属第三医院(常州市第一人民医院)血液净化中心,江苏省213003

出　　处：《江苏医药》2015年第20期2426-2428,2432,共4页Jiangsu Medical Journal

摘　　要：目的探讨维持性血液透析患者外周血CD14+CD16+单核细胞的表达。方法流式细胞术检测50例维持性血液透析患者(A组)和30例正常对照者(C组)外周血CD14+CD16+单核细胞百分比,ELISA法检测两组血清IL-6水平。结果两组血尿素氮、血清肌酐、肾小球滤过率、血红蛋白和三酰甘油比较有统计学差异(P<0.05)。A组CD14+CD16+单核细胞表达高于C组[(8.86±0.81)%vs.(2.84±1.81)%](P<0.01)。A组CD14+CD16+单核细胞表达与IL-6和三酰甘油均呈正相关(r=0.83和0.45,P<0.01)。结论维持性血液透析患者外周血CD14+CD16+单核细胞参与了机体免疫炎症反应;其机制可能与IL-6介导的信号转导和转录激活因子(STAT)通路有关。Objective To investigate the expression of CD14＋CD16＋monocytes in peripheral blood of patients undergoing maintenance hemodialysis.Methods The expression of CD14＋CD16＋monocytes in peripheral blood was detected by flow cytometry in 50 patients undergoing maintenance hemodialysis（group A）and 30 healthy controls（group C）.Serum level of IL-6in two groups was detected by ELISA.Results There were significant differences in blood urea nitrogen,serum creatinine,glomerular filtration rate,hemoglobin and triglyceride between two groups（P〈0.05）.The expression of CD14＋CD16＋monocytes in group A was higher than that in group B[（8.86±0.81）%vs.（2.84±1.81）%]（P〈0.01）.The expression of CD14＋CD16＋monocytes in group A was positively correlated to that of IL-6and triglyceride（r=0.83 and 0.45,P〈0.01）.Conclusion CD14＋CD16＋monocytes are involved in immunoinflammatory responses in the patients undergoing maintenance hemodialysis,which may be related to the STAT pathway mediated by IL-6.

关 键 词：维持性血液透析 单核细胞 

分 类 号：R459[医药卫生—治疗学]