一种新的西瓜果斑病种子带菌检测方法  

A New Pathogen Detection Methodon for Watermelon Seeds

作　　者：马艺荞 惠长敏 王秀峰[1] 王利波[1] 陈莹[1] 王永卓[1] 王健鹂 

机构地区：[1]吉林省蔬菜花卉科学研究院,长春130033

出　　处：《长江蔬菜》2015年第20期91-94,共4页Journal of Changjiang Vegetables

基　　金：国家西甜瓜产业技术体系(CARS-26-26);吉林省西瓜细菌性果斑病快速检测及综合防治技术研究(20130102047JC)

摘　　要：通过采用病原菌分离、培养基富集培养与PCR技术建立了一套西瓜果斑病菌检测方法。结果表明,MOPS缓冲液对西瓜种子所带病菌分离效果最佳,且利用WFB1/WFB2引物对富集到的病菌进行PCR检测,都产生了预期360 bp片段;对BFB纯菌液和模拟带菌种子浸提液的最低检测限为1.0×102 cfu/m L。利用OD值法结合平板涂布记菌落数方法绘制西瓜细菌性果斑病病原菌浓度梯度标准曲线:Y=3.984 1x+0.088 1,R2=0.9991(Y×108cfu/m L)。该检测方法的建立,为西瓜生产中果斑病防治提供了重要技术支持。In this study, we established a separation-enrichment-PCR detection system. The results showed the MOPS so-lution was the best one for detecting the seeds carrying BFB, the enrichment of bacteria was amplified by the primer sets WFB1/WFB2 and all produced 360 bp specific fragments; the method assay had a lowest detection limit of 1.0×10^2 cfu/mL. We drew a watermelon bacterial fruit blotch of standard curve： Y=3.9841x＋0.0881, R2=0.9991 （Y×10^8cfu/mL）. This method can greatly in-crease the precision of this detection and provide an important technical support for the prevention and control of BFB.

关 键 词：西瓜 细菌性果斑病 种子 检测 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]