男性不育患者精液中金黄色葡萄球菌及其毒力基因对精子制动活性研究  

Spermatozoal immobilization ability and virulence genes of Staphylococcus aureus isolated from the semen of infertile men

作　　者：李斌[1] 杨旭[2] 叶建中[1] 陈华乐[1] 侯渊博 杜佳[1] 周铁丽[1] 

机构地区：[1]温州医科大学附属第一医院医学检验中心,浙江温州325035 [2]温州医科大学附属第一医院生殖医学中心,浙江温州325035 [3]温州医科大学检验医学院/生命科学学院,浙江温州325035

出　　处：《中华男科学杂志》2015年第10期881-886,共6页National Journal of Andrology

基　　金：浙江省自然科学基金(LY14H190005);浙江省卫生高层次创新人才培养工程项目(浙卫发[2012]241号)~~

摘　　要：目的:研究男性不育患者精液中金黄色葡萄球菌对精子活动力的影响,并探讨金黄色葡萄球菌毒力基因与其精子制动活性之间的关系。方法:收集589例男性不育患者精液中分离的非重复金黄色葡萄球菌60株,通过计算机辅助精子分析系统检测金黄色葡萄球菌对健康男性精液中精子活力的影响,筛选出可以使精子活力明显下降的菌株,采用普通PCR方法对金黄色葡萄球菌菌株进行相关毒力基因检测。结果:60株金黄色葡萄球菌菌株中有17株可以引起精子活力明显下降(P<0.05);菌株携带的毒力基因主要包括hlg、scn、cna、hlb及clf A,阳性率分别为33.3%、23.3%、20%、20%及18.3%。其他还包括ica A、fnb A、tst、seb、hld、eta、sea。其中,精子制动阳性组的scn基因携带率(47.1%)高于精子制动阴性组(14%),差异具有统计学意义(P<0.05);其余毒力基因在两组间携带率均无明显差异(P>0.05)。结论:男性生殖系统金黄色葡萄球菌感染能导致精子活力下降,可能与葡萄球菌补体抑制剂编码基因scn相关。Objective：To investigate the impact of Staphylococcus aureus from infertile men on sperm motility and the relationship between virulence genes and the activity of spermatozoal immobilization.Methods：We collected 60 strains of non-repeated Staphylococcus aureus from the semen of 589 infertile males and analyzed the influence of Staphylococcus aureus on sperm motility using the computer-aided sperm analysis system.We selected the strains that apparently decreased sperm motility and detected their virulence genes by PCR.Results：Sperm motility was significantly decreased in 17 of the 60 strains of Staphylococcus aureus（P 〈0.05）.The main virulence genes in these strains were hlg（33.3%）,scn（23.3%）,cna（20%）,hlb（20%）,and clf A（18.3%）,others including ica A,fnb A,tst,seb,hld,eta and sea.The scn gene carriers accounted for 47.1% in the spermatozal immobilization positive group,significantly higher than 14% in the negative group（P 〈0.05）.No statistically significant differences were found in the percentages of the carriers of the other virulence genes between the two groups（P 〉0.05）.Conclusion：Infections of Staphylococcus aureus in male reproductive system can lead to the decrease of sperm motility,which may be associated with the Staphylococcus complement inhibitor encoding gene scn.

关 键 词：金黄色葡萄球菌 感染 男性不育 精子活力 毒力基因 

分 类 号：R446.5[医药卫生—诊断学] R691.3[医药卫生—临床医学]