盐酸戊乙奎醚对新生大鼠内毒素性急性肺损伤时TLR4 mRNA表达的影响  

Effects of penehychdine hydrochloride on TLR4 mRNA expression during endotoxin-induced acute lung injury in neonatal rats

作　　者：王涛[1] 魏晓永[1] 刘博[1] 赵军博[1] 姜丽华[1] 李黎[1] 张建辉[1] 董正华[1] 邵勇[1] 

机构地区：[1]郑州大学第三附属医院麻醉科,450052

出　　处：《中华麻醉学杂志》2015年第7期883-885,共3页Chinese Journal of Anesthesiology

摘　　要：目的评价盐酸戊乙奎醚对新生大鼠内毒素性急性肺损伤时Toll样受体4（TLR4）mRNA表达的影响。方法清洁级健康雄性Wistar大鼠30只，体重12～18g，7日龄。采用随机数字表法分为3组（n=10）：生理盐水组（NS组）、急性肺损伤组（ALI组）和盐酸戊乙奎醚组（PH组）。采用腹腔注射内毒素5mg／kg的方法制备大鼠内毒素性急性肺损伤模型，PH组腹腔注射盐酸戊乙奎醚0．5mg／kg后30min制备模型，NS组给予等容量生理盐水。于注射内毒素后dh时处死大鼠取肺组织，采用RT—PCR法检测TLR4mRNA表达，ELISA法测定TNF-α、IL-1β和IL-10的含量，光镜下观察肺组织病理学结果。结果与NS组比较，ALl组和PH组肺组织TLR4mRNA表达上调，TNF-α、IL-1β和IL-10含量升高（P〈0．05）；与ALl组比较，PH组肺组织TLR4mRNA表达下调，TNF-α、IL-1β和IL-10含量降低（P〈0．05）。PH组肺组织病理学损伤较ALI组减轻。结论盐酸戊乙奎醚通过下调肺组织TLR4mRNA表达，抑制炎性反应，从而减轻新生大鼠内毒索性急性肺损伤。Objective To evaluate the effects of penehychdine hydrochloride on Toll-like receptor 4 （TLR4） mRNA expression during endotoxin-induced acute lung injury （ALI） in neonatal rats. Methods Thirty pathogen-free male Wistar rats, aged 7 days, weighing 12- 18 g, were randomly divided into 3 groups （n= 10 each） using a random number table： normal saline group （group NS）, ALl group, and penehyehdine hydroehloride group （group PH）. ALl was induced with intraperitoneal endotoxin 5 mg/kg. In group PH, penehychdine hydrochloride 0.5 mg/kg was injected intraperitoneally at 30 rain before ALl, while the equal volume of normal saline was given instead in group NS. At 4 h after endotoxin injection, the rats were sacrificed, and lungs were removed for determination of TLR4 mRNA expression （using real-time reverse transcriptase polymerase chain reaction） , and contents of tumor necrosis factor-alpha （TNF-α） , interleukin-1 beta （IL-1β） , and IL-10 in the lung tissues （by enzyme-linked immunosorbent assay） , and for examination of the pathologic changes of lung （with electron microscope）. Results Compared with group NS, TLR4 mRNA expression was significantly up-regulated, and contents of TNF-α, IL-1β, and IL-10 were increased in ALI and PH groups. Compared with group ALI, TLR4 mRNA expression was significantly down-regulated, and contents of TNF-α, IL-1β, and IL-10 were decreased in group PH. The pathologic changes of lung were significantly mitigated in group PH compared with group ALI. Conclusion Penehychdine hydrochloride inhibits inflammatory responses through down-regulating TRL4 mRNA, thus reducing endotoxin-induced ALI in neonatal rats.

关 键 词：胆碱能拮抗剂 婴儿 新生 内毒素血症 呼吸窘迫综合征 成人 TOLL样受体4 

分 类 号：R722.1[医药卫生—儿科]