铁皮石斛龟苓膏对免疫抑制小鼠免疫功能影响的实验研究  

Experimental Study on Effects of Tiepi Shihu Guiling Gao( 铁皮石斛龟苓膏) on Immunologic Function of Immunosuppressive Mice

作　　者：戴世杰[1] 何潆 李哲明[1] 周小杰[1] 李昌煜[1] 

机构地区：[1]浙江中医药大学,杭州310053

出　　处：《中国中医药科技》2015年第5期496-497,505,共3页Chinese Journal of Traditional Medical Science and Technology

基　　金：浙江省大学生科技创新活动项目(2013124110060)

摘　　要：目的:探讨铁皮石斛龟苓膏对免疫抑制小鼠免疫功能的影响。方法:实验以环磷酰胺致免疫抑制小鼠为研究对象,分别按铁皮石斛龟苓膏低、中、高剂量灌胃给药,观察铁皮石斛龟苓膏对免疫抑制小鼠血清溶血素水平、迟发型变态反应、血清IL-4和IFN-γ含量以及单核-巨噬细胞吞噬功能、外周白细胞数和淋巴细胞数的影响。结果:与环磷酰胺组比较,铁皮石斛龟苓膏各剂量组均提高免疫抑制小鼠血清溶血素水平(P<0.01)、IL-4和IFN-γ含量(P<0.05)、外周白细胞数和淋巴细胞数(P<0.05);中、高剂量组均提高小鼠迟发型变态反应(P<0.05);中剂量组增强小鼠单核-巨噬细胞吞噬功能(P<0.05)。结论:铁皮石斛龟苓膏具有提高免疫抑制小鼠免疫功能的作用。Objective： To study effects of Tiepi Shihu Guiling Gao on immunologic function of immunosuppressive mice. Methods： The cyclophosphamide- induced immunosuppressed mice were taken as models. The mice were fed with Tiepi Shihu Guiling Gao at the dose of 0. 75,1. 5,3（ g / kg） respectively,observing the effects of Tiepi Shihu Guiling Gao on level of serum hemolysin,delayed hypersensitivity,serum IL- 4 and IFN- γ levels,monocyte- macrophage phagocytosis,peripheral white blood cell count and lymphocyte count in immunosuppressive mice. Results： To compare with cyclophosphamide group,each dose groups of Tiepi Shihu Guiling Gao could increase serum hemolysin level（ P〈0. 01）,raise serum IL- 4 and IFN- γ levels（ P〈0. 05）,increase the number of peripheral leukocytes and lymphocytes（ P〈0. 05）; medium and high dose groups could enhance delayed type hypersensitivity（ P〈0. 05）; high dose group could enhance monocyte- macrophage phagocytosis（ P〈0. 05）. Conclusion： Tiepi Shihu Guiling Gao could enhance immune function of immunosuppressive mice.

关 键 词：免疫抑制 铁皮石斛龟苓膏 免疫功能 小鼠 

分 类 号：R285.5[医药卫生—中药学]