外周血标本处理方法和时间影响可溶型CD100的水平  

Effects of different processing methods and durations of blood specimens on the level of soluble CD100 in peripheral blood

作　　者：刘志佳[1,2] 张春梅[3] 李琦[3] 肖漓[2] 孔祥瑞[2] 许晓东[2] 石炳毅[2] 

机构地区：[1]解放军医学院泌尿外科,北京100853 [2]解放军第309医院全军器官移植研究所,北京100091 [3]第四军医大学免疫学教研室,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2015年第10期1375-1377,1382,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家高科技研究发展计划(2012AA021002)

摘　　要：目的观察血清和血浆标本不同处理方法和时间对外周血可溶型CD100(sCD100)水平的影响。方法用未加促凝剂血清采血管、含硅化涂层促凝管以及肝素抗凝管、乙二胺四乙酸(EDTA)抗凝管和枸橼酸钠抗凝管,分别在凝血1、4、8 h,分别收集2种血清收集管内血清标本。采用ELISA检测标本中sCD100水平,比较不同标本和时间处理对sCD100检测水平的影响。结果不同抗凝剂处理后的血浆标本中,sCD100检测结果无明显差异。血清标本sCD100水平明显高于血浆标本。使用促凝剂血清sCD100水平高于无促凝剂血清sCD100水平。在室温条件下,血清标本随着凝血时间的延长,sCD100水平逐渐升高。结论血清中sCD100水平显著高于血浆,处理时间延长可引起血清sCD100水平进一步增高。在定量检测白细胞和血小板膜分子的可溶型标志物时,应注意标本收集过程中的标准化。Objective To investigate the effects of different processing methods and durations of blood specimens on the level of soluble CD100 （sCD100） in peripheral blood. Methods We included a total of 15 healthy individuals without any significant signs or symptoms of microbial infection. Blood samples were collected in tubes with and without additives, as well as tubes containing citrate, EDTA and heparin, respectively, and processed for different durations of time. The serum samples were allowed to clot for 1 hour, 4, 8 hours after venipuncture before centrifugation. Then the sCD100 levels were measured with ELISA. Results Serum sCD100 levels were higher than those in plasma, and no significant differences were observed among the citrate, EDTA or heparin treated plasma groups. The sera in the tubes without additives showed lower levels of sCD100 than those in the tubes with additives. The concentrations of sCD100 in sera increased with the prolonged processing time in room temperature. Conclusion The measurement of sCD100 in blood samples may be interfered by the in vitro release from platelets. Thus, pre-treating methods for the collection of serum or plasma samples are critical in the assessment of sCD100 concentrations and should be carefully considered for the measurement of this important biomarker in both basic and clinical studies.

关 键 词：可溶型CD100 检测 血清 血浆 酶联免疫吸附法 

分 类 号：R392.4[医药卫生—免疫学] R392.12[医药卫生—基础医学]