呼吸道合胞病毒毛细支气管炎大鼠肺组织视黄酸受体相关孤核受体γt水平增加  

The level of RORγt increases in rat lung tissues of bronchiolitis caused by respiratory syncytial virus

作　　者：高萌[1] 吴福玲[2] 李营营[2] 石涛[2] 田丽君[1] 韩亭亭[1] 王海英[1] 

机构地区：[1]滨州医学院临床学院儿科学教研室 [2]滨州医学院附属医院儿科,山东滨州256003

出　　处：《细胞与分子免疫学杂志》2015年第11期1492-1496,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：山东省医药卫生科技发展计划项目(2013WS0312)

摘　　要：目的研究视黄酸受体相关孤核受体γt(RORγt)在呼吸道合胞病毒(RSV)毛细支气管炎大鼠肺组织和外周血单个核细胞(PBMC)中的水平及其意义。方法按随机对照原则将SD大鼠分为正常组和RSV毛细支气管炎组,滴鼻法成功建立RSV毛细支气管炎模型,通过HE染色观察肺组织病理改变,ELISA检测血浆中白细胞介素17(IL-17)、IL-23的水平,实时荧光定量PCR检测肺组织和PBMC中RORγt mRNA的表达,Western blot法检测各组肺组织中RORγt蛋白的表达。结果与正常组相比,RSV感染组肺间质充血水肿严重伴炎性细胞浸润,肺泡间隔增宽,支气管塌陷变形;RSV感染组大鼠肺组织和PBMC中RORγt mRNA表达上调,血浆IL-17、IL-23显著升高;RSV感染组大鼠肺组织RORγt蛋白水平增加。结论 RORγt在RSV毛细支气管炎大鼠肺组织中增加。Objective To study the level of retinoic acid receptor-related orphan receptor γt （RORγt） in rat lung tissues of bronchiolitis caused by respiratory syncytial virus （RSV） and its implication. Methods The rats were randomly divided into normal group and bronchiolitis group. After the model of bronchiolitis was established successfully by nasal dripping, the pathological changes of lung tissues were detected by HE staining; the plasma levels of interleukin 23 （IL-23）, IL-17 were detected by ELISA; the level of RORγt mRNA in lung tissues and peripheral blood mononuclear cells （PBMCs） were detected by real-time quantitative PCR; the level of RORγt protein in lung tissues was examined by Western blotting. Results Compared with the normal group, the rats with bronchiolitis presented with pulmonary interstitial hyperemia and edema, more inflammatory cell infiltration, wider alveolar septa and bronchial collapse and deformation. Compared with the normal group, the level of RORγt mRNA in the lung tissues and PBMCs increased in rats with bronchiolitis. The level of RORγt protein in lung tissues and the plasma levels of IL-23 and IL -17 were higher in rats with bronchiolitis than in normal rats. Conclusion The level of RORγt was elevated in the lung tissues of rats with RSV-induced bronchiolitis.

关 键 词：RORΓT RSV 毛细支气管炎 

分 类 号：R562.21[医药卫生—呼吸系统] R392-33[医药卫生—内科学]