CXC趋化因子受体3的单克隆抗体抑制体外培养的MCF-7细胞和HepG2细胞的增殖及迁移  

CXCR3 monoclonal antibody inhibits the proliferation and migration of MCF-7 cells and HepG2 cells in vitro

作　　者：朱玉强[1] 王志遥 朱莹[1] 王婧[1] 蔡磊[1] 沈辉[1] 孔永[1] 邱玉华[1] 

机构地区：[1]苏州大学医学部免疫学系,江苏苏州215123

出　　处：《细胞与分子免疫学杂志》2015年第11期1544-1548,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81373236)

摘　　要：目的研究CXC趋化因子受体3(CXCR3)单克隆抗体(mAb)对MCF-7人乳腺癌细胞和HepG2人肝癌细胞体外增殖及迁移的影响。方法诱生腹水法制备CXCR3 m Ab;流式细胞术筛选高表达CXCR3的MCF-7细胞和HepG2细胞;MTT法检测CXCR3 mAb对MCF-7细胞和HepG2细胞增殖及干扰素诱导的T细胞α趋化因子(I-TAC)对MCF-7细胞和HepG2细胞促增殖的影响;TranswellTM法研究CXCR3 mAb对MCF-7细胞和HepG2细胞迁移及I-TAC对MCF-7细胞和HepG2细胞促迁移的影响。结果 MCF-7细胞和HepG2细胞CXCR3的阳性表达率分别为83.5%和96.2%;50μg/m L CXCR3 m Ab能够显著的抑制MCF-7细胞和HepG2细胞的增殖并抑制I-TAC对MCF-7细胞和HepG2细胞的促增殖作用;50μg/m L CXCR3 m Ab可抑制MCF-7细胞和HepG2细胞的迁移,并抑制I-TAC对MCF-7细胞和HepG2细胞促迁移作用。结论 CXCR3 mAb能够显著地抑制高表达CXCR3的肿瘤细胞的增殖及迁移。Objective To study the effects of chemokine （C-X-C motif） receptor 3 （CXCR3） monoclonal antibody （mAb） on the proliferation and migration of MCF-7 and HepG2 cells in vitro. Methods Ascites of CXCR3 mAb was prepared at first. MCF-7 and HepG2 cells with high expressions of CXCR3 were screened by flow cytometry. MTT assay was used to detect the effects of CXCR3 mAb on the proliferation of MCF-7 and HepG2 cells in vitro in the absence/presence of interferoninducible T-cell alpha chemoattractant （I-TAC）. Transwell^TM assay was performed to investigate the effects of CXCR3 mAb on the migration of MCF-7 and HepG2 cells in vitro in the absence/presence of I-TAC. Results The expression rate of CXCR3 on MCF-7 and HepG2 cells were 83.5% and 96.2%, respectively. 50 μg/mL CXCR3 mAb significantly inhibited the proliferation and migration of MCF-7 and HepG2 cells, and also inhibited the promoting effect of I-TAC on the proliferation and migration of MCF-7 and HepG2 cells in vitro. Conclusion CXCR3 mAb can significantly inhibit the proliferation and migration of the tumor ceils highly expressing CXCR3 in vitro.

关 键 词：CXC趋化因子受体3(CXCR3) 单克隆抗体 肿瘤 增殖 迁移 

分 类 号：R392.11[医药卫生—免疫学] Q279[医药卫生—基础医学]