人包皮成纤维细胞支持生长的人胚胎干细胞小鼠体内分化全能性观察  

Totipotency analysis of human embryonic stem cells supported by human postnatal foreskin fibroblasts in mice

作　　者：米蔷[1] 何志旭[2,3] 

机构地区：[1]上海交通大学附属医院上海儿童医学中心,上海200271 [2]贵阳医学院附属医院 [3]贵阳医学院组织工程及干细胞中心

出　　处：《山东医药》2015年第38期24-26,共3页Shandong Medical Journal

基　　金：上海青年医师培养资助计划项目(EY21.001)

摘　　要：目的观察用人包皮成纤维细胞(h PFF)支持生长的人胚胎干细胞(h ESC)的体内全能性。方法将第20代h PFF支持生长的h ESC和h PFF分别接种到2只严重联合免疫缺陷的小鼠(SCID)小鼠体内,分别为观察组和对照组,观察5周内两组小鼠一般状态及是否有畸胎瘤形成。取观察组小鼠畸胎瘤制作组织学切片,验证其是否包含了源于全部3个胚层的各种分化细胞。结果观察组SCID小鼠接种后第5天即可在接种部位触到包块,包块进行性增大。观察组小鼠包块理组织学检查显示,该肿块包含3个胚层的组织结构,具备畸胎瘤的病理组织学特点。结论用h PFF支持生长的h ESC有体内分化全能性。Objective To observe the totipotency analysis of human embryonic stem cells（ h ESCs） supported by human postnatal foreskin fibroblasts（ h PFFs）. Methods The h ESCs of the 20 th passage supported by h PFF and h PFF were respectively injected into the hind legs of the severe combined immunode？ cient mice（ SCID） and taken as the observation and control group. After 5-week,we observe the general state and teratoma formation of the two mice. The teratoma was taken from the observation group for the histological analysis to investigate whether there were different kinds of differentiated cells derived from three germ layers. Results We touched the masses in the vaccination site of SCID mice on the fifth day after inoculation,and the masses increased progressively. Five weeks later,we killed the mice to strip the tumor,and then they received the histologic examination. It showed that the mass contained three germ layers＇ structures with the histologic characteristics of teratoma. Conclusions The h ESCs supported by h PFFs have totipotency.

关 键 词：胚胎干细胞 成纤维细胞 饲养层 人类 畸胎瘤 小鼠 

分 类 号：R318.5[医药卫生—生物医学工程]