载药磁纳米微粒在荷瘤小鼠体内的靶向性实验研究  

Targeting experiment of drug-bearing magnetic nano-particle in tumor-bearing mice

作　　者：胡瑛[1] 郝欢[2] 秦庆[1] 于增国[2] 

机构地区：[1]解放军210医院检验科,辽宁大连116021 [2]大连大学医学院,辽宁大连116622

出　　处：《医疗卫生装备》2015年第10期14-16,20,共4页Chinese Medical Equipment Journal

基　　金：2014辽宁省(国家)大学生创新创业训练计划项目(201411258)

摘　　要：目的 :应用高效液相色谱法考察在外磁场协同下载阿霉素金磁复合微粒于荷瘤小鼠体内靶向分布特性。方法 :建立小鼠肝细胞癌动物模型,并分成空白对照组、单纯磁阿霉素组和磁阿霉素加磁场组。空白对照组经尾缘静脉注射生理盐水,单纯磁阿霉素组和磁阿霉素加磁场组分别注射同等剂量的载阿霉素金磁复合微粒溶液,注射后于磁阿霉素加磁场组肿瘤靶区体表施加磁场固定(5 000 Gs、1 h)。然后移去磁场并立即处死各组小鼠,分别采集血液并完整摘取心、肝、肾和肿瘤组织标本。应用高效液相色谱法测定各组小鼠血液及各组织中阿霉素浓度,评价其在小鼠体内的靶向性。结果:经外置磁场固定作用后,靶区肿瘤组织中阿霉素浓度明显高于其他非靶区组织,而其他非靶区组织中(除心脏外)阿霉素浓度与空白对照组相当。在没有外置磁场作用时,阿霉素则在体内各组织中呈弥散性分布,但肝脏和肿瘤组织中阿霉素浓度较高。结论:磁纳米微粒作为药物载体具有明显的磁控靶向性,能将药物有效富聚于靶区肿瘤组织中。磁靶向药物有望成为最有效的抗肿瘤药物新剂型而用于临床。Objective To investigate the targeting distribution of adriamycin-bearing gold magnetic particle in the tumorbearing mice by using high-performance liquid chromatography and external magnetic field. Methods Mouse hepatocellular carcinoma models were established and then divided into a control group（group A）, a group with adriamycin-bearing magnetic nano-particles（group B） and a group with external field and adriamycin-bearing magnetic nano-particles（group C）. The mice in group A were administrated with physiological saline in the tail veins, and the ones in group B and C were infused with the solution of adriamycin-bearing gold magnetic particles. Some external magnetic field（5 000 Gs, 1 h）was posed on the target areas of the mice in group C and then removed after a while. All the mice in the three groups were killed, and the blood and the specimens of the hearts, livers, kidneys and tumor tissues were collected. The concentrations of adriamycin in the blood and tissues were detected with high-performance liquid chromatography to evaluate in vivo targeted distribution in mice. Results External field made the adriamycin concentrations in the targeted tumor areas significantly higher than those in non-targeted areas, and the non-targeted areas except the heart had the concentrations equivalent to those in the control group. When the external field was absent, adriamycin had diffused distribution in the tissues while high concentrations in the liver and tumor tissues. Conclusion Magnetic nano-particle shows magnetically controlled targeting when used as drug-bearing carrier and can concentrate the drug in the targeted tumor tissues. Magnetically targeted drug may be popularized clinically as a new-type antineoplastic agent.

关 键 词：磁靶向给药系统 肝细胞癌 阿霉素 金磁复合微粒 高效液相色谱法 

分 类 号：R965[医药卫生—药理学] TB383.1[医药卫生—药学]