益气化瘀方对大鼠腰神经根压迫损伤后背根节神经元TRPV4基因表达的影响  

Influence of Yiqi Huayu Formula on TRPV4 Gene Expression of Dorsal Root Ganglion Neurons of Lumbar Nerve Root Compression Injury in Rats

作　　者：唐占英[1] 杨强玲[1] 胡志俊[1] 崔学军[2] 刘丹[1] 林婕[1] 王拥军[2] 施杞[2] 

机构地区：[1]上海中医药大学附属龙华医院,上海200032 [2]上海中医药大学脊柱病研究所,上海200032

出　　处：《辽宁中医杂志》2015年第10期2021-2023,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金青年科学基金项目(81102605);上海中医药大学第四批后备业务专家计划;上海市吉林新星计划(ZYSNXD011-RC-XLXX-20130052);上海市科委基础研究项目(12ZR1431900)

摘　　要：目的:观察益气化瘀方对压迫损伤后不同时间点大鼠DRG神经元TRPV4基因表达的影响。方法:大鼠随机分为假手术组10只、模型组40只、中药组40只。模型组和中药组又根据取材、给药时间点的不同分为3 d组、7 d组、14 d组、28 d组,每组10只。用Von Frey法测试大鼠造模后50%撤足阈值;应用RT-PCR法检测大鼠DRG神经元TRPV4基因表达情况。结果:造模后,各模型组大鼠与假手术组相比50%撤足阈值均有显著性降低;中药干预后,在中药7d组、14 d组和28 d组大鼠50%撤足阈值均显著高于相应时间点各模型组。模型组各时间点TRPV4mRNA的表达与假手术组相比显著增高。中药干预后第3天、第7天和第14天组TRPV4mRNA的表达仍高于假手术组,而中药28 d组TRPV4mRNA的表达与假手术组相比无显著性差异。在第7天、第14天和第28天,中药干预组和模型组各时间点TRPV4mRNA表达相比均有显著性差异。结论:益气化瘀方可能通过下调TRPV4基因表达从而影响神经根压迫损伤后根性神经痛的产生。Objective： To observe the influence of Yiqi Huayu Formula on TRPV4 gene expression of dorsal root ganglion neurons of lumbar nerve root compression injury in rats. Methods： The rats were randomly divided into sham operation group（ 10rats）,model group（ 40 rats） and TCM group（ 40 rats）. The model and TCM groups were divided into 3 d group,7 d group,14 d group,14 d group and 28 d group according to materials,administration time,10 in each. Von Frey method was used to detect the50% foot- withdrawing threshold and RT- PCR to detect the TRPV4 gene expression. Results： After modeling,compared with the sham operation group,the model groups＇ 50% foot- withdrawing threshold was significantly decreased. After TCM intervention,TCM 7 d,14 d and 28 d groups＇ 50% foot- withdrawing threshold was significantly higher than that of the model groups. Compared with the sham operation group,model groups＇ TRPV4 mRNA expressions at different time points were significantly increased. TCM 3 d,7 d and 14 d groups＇ TRPV4 mRNA expressions were higher than that of the sham operation group. TCM 28 d group＇s RPV4 mRNA expression had no difference compared with that of sham operation group. On 7,14 and 28 d,TCM and model groups＇ TRPV4 mRN expressions had significant difference. Conclusion： Yiqi Huayu Formula may down- regulate the TRPV4 gene expression to influence the nervous pain due to lumbar nerve root compression injury.

关 键 词：益气化瘀方 背根节 痛觉过敏 TRPV4 

分 类 号：R322.1[医药卫生—人体解剖和组织胚胎学]