高压氧预处理对急性高海拔暴露体力负荷人体应激反应的作用  

Effects of hyperbaric oxygen preconditioning on human stress responses during acute exposure to high altitude

作　　者：李洋洋[1] 石路[2] 吴楠宁[3] 刘俊松[4] 张延猛[1] 张明月[4] 吴岩印[4] 牟杰[4] 刘洪涛[1] 

机构地区：[1]上海交通大学海洋水下工程科学研究院,200231 [2]上海交通大学一千叶大学国际合作研究中心 [3]中国人民解放军总装备部电子信息基础部技术基础局 [4]中国人民解放军南京军区杭州疗养院海勤疗养区

出　　处：《中华劳动卫生职业病杂志》2015年第10期731-734,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：中国人民解放军总装备部装备技术基础项目（YXSJD20132BZ01）

摘　　要：目的探讨高压氧预处理（HBOP）对急性高海拔暴露体力负荷人体应激反应的作用及可能机制。方法对8名男性受试者进行3、5和7d的HBOP，然后急性暴露于模拟4000m高海拔的低氧环境。安静状态分为3组：海平对照组、模拟高海拔组和HBOP干预5d组；负荷后分为4组：海平对照组、模拟高海拔组、HBOP干预3d组和7d组。在HBOP前后作自身对比体力负荷试验，并于运动前后检测血浆多巴胺（DA）、肾上腺素（E）、去甲肾上腺素（NE）和促肾上腺皮质激素（ACTH）水平。体力负荷方式：踏阶运动25次／min，5min，阶梯高30cm，用节拍器控制踏阶速度和计时，属中等体力劳动。结果急性高海拔暴露后机体在安静和体力负荷后的血浆DA、E、NE和ACTH水平均较海平对照组明显升高，差异均有统计学意义（均P〈0．05）；HBOP干预5d组再次急性高海拔暴露后人体安静时血浆DA、E、NE和ACTH水平均较模拟高海拔组明显升高，差异均有统计学意义（均P〈0．01）；HBOP干预3、7d组再次急性高海拔暴露体力负荷后的血浆DA、E、NE和ACTH水平（除HBOP干预3d组的ACTH）均较模拟高海拔组明显升高，差异均有统计学意义（均P〈0．01）。结论HBOP可以提高机体血浆DA、E、NE和ACTH表达水平，从而加快机体内环境稳态建立新的平衡以适应急性高原低氧环境。Objective To explore the effects of hyperbaric oxygen preconditioning （HBOP） on human stress responses during acute exposure to high altitude and the possible mechanism. Methods Eight male subjects were treated with HBOP for 3, 5, and 7 days, followed by acute exposure to hypoxia simulating an altitude of 4,000 m. Subjects at rest were divided into sea-level control group, simulated high-altitude group, and 5-day HBOP intervention group, while subjects after physical load were divided into sea-level control group, simulated high-altitude group, 3-day HBOP intervention group, and 7-day HBOP intervention group. The physical load test was performed for each subject before and after HBOP, and the plasma levels of dopamine （DA）, epinephrine （E）, norepinephrine （NE）, and adrenoeorticotropic hormone （ACTH） were determined before and after exercise. The physical load test was performed by stepping up on to a 30 cm-high stepping stool at a rate of 25/min for 5 minutes, which was a type of moderate physical exercise. The stepping rate and timing were controlled by a metronome. Results The levels of DA, E, NE, and ACTH at rest and after physical load were significantly higher in subjects acutely exposed to high altitude than in the sea-level control groups （all P〈0.05）. Moreover, the levels of DA, E, NE, and ACTH at rest were significantly higher after acute exposure to high altitude in the 5-day HBOP intervention group than in the simulated high-altitude group （all P〈0.01）. Except for the ACTH level in the 3-day HBOP intervention group, the levels of DA, E, NE, and ACTH after physical load were significantly higher after acute exposure to high altitude in the 3-day and 7-day HBOP intervention groups than in the simulated high-altitude group （all P〈0.01）. Conclusion HBOP can elevate the plasma expression of DA, E, NE, and ACTH, and then speed up the establishment of a new balance of homeostasis to adapt to the acute hypoxia at high altitude.

关 键 词：高压氧 急性高海拔暴露 体力负荷 应激 

分 类 号：R82[医药卫生—临床医学]